| 引用本文:邓 姝 沈建平 沈一平 林圣云 胡致平 郑智茵 周郁鸿.维生素K3增强三氧化二砷诱导多发性骨髓瘤细胞凋亡的实验研究[J].世界中医药,2012,7(5):. |
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| 维生素K3增强三氧化二砷诱导多发性骨髓瘤细胞凋亡的实验研究 |
| VK3 Enhances Apoptosis of Multiple Myeloma RPM I 8226 Cells Induced by Arsenic Trioxide |
| 投稿时间：2012-06-01 |
| DOI： |
| 中文关键词: 维生素K3 三氧化二砷 多发性骨髓瘤 凋亡 |
| English Keywords:VK3 As2O3 Multiple Myeloma Apoptosis |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨维生素K3对三氧化二砷(As2O3)诱导人多发性骨髓瘤(MM)细胞株RPMI 8226细胞凋亡的作用及生长抑制。方法：采用四甲基噻唑氮蓝比色法检测As2O3联合维生素K3对人多发性骨髓瘤(MM)细胞株RPMI 8226细胞生长抑制作用;流式细胞术检测As2O3联合维生素K3对RPMI8226细胞周期和凋亡率的影响。结果：单用As2O3对RPMI 8226细胞的生长有抑制作用，其作用呈时间和剂量依赖性；与单用As2O3相比，As2O3联合维生素K3作用于RPMI 8226细胞48h后，细胞凋亡率显著增加(P<0.05)，G0/G1期细胞比例升高，S期减低，并有凋亡峰。结论：维生素K3可以增强As2O3对RPMI8226细胞诱导凋亡作用。 |
| English Summary: |
| To investigate the effect of VK3 on arsenic trioxide induced apoptosis of multiple myeloma RPMI8226 ce11s and the molecular mechanisms.Methods:Growth inhibition of RPMI8226 cells exposed to Vitamin K3 Combination with As2O3 was analyzed by MTT assay，Annexin V labeling.Results:As2O3 alone could inhibit the growth and induce apoptosis of RPMI 8226 cells，whil e As2O3 inhibited the proliferation of RPMI8226 cells in a time and dose-dependent manner.Compared with As2O3 treatment alone，growth inhibition was enhanced by combination therapy with As2O3 and VK3 (P<0.05)，Annexin V staining showed the earlier apoptotie cells.Flow cytometry showed that the proportion of G0/G1 cells was increased and cell proportion in phase was decreased with appearance of apoptosis peak.Conclusion:VK3 enhances the apoptosis induced by arsenic trioxide in RPMI8226 cells. |
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