| 引用本文:周洪伟 郝丽静 杨 勇 张 林 杨 桃 周 坤 吴晓丹.星点设计-效应面法优化玄参提取工艺[J].世界中医药,2012,7(5):. |
|
| 星点设计-效应面法优化玄参提取工艺 |
| Optimization of Extraction Process Based on Central Composite Design and Response Surface Methodology for Radix Scrophulariae |
| 投稿时间：2012-05-24 |
| DOI： |
| 中文关键词: 玄参 哈巴苷 哈巴俄苷 提取工艺 星点设计 效应面法 |
| English Keywords:Radix scrophulariae Harpagide Harpagoside Extraction process Central composite design Response surface methodology |
| 基金项目:国家自然科学基金青年科学基金资助项目(编号：81001495/H2705)；北京中医药大学复方中药制药研究创新团队资助项目(编号：2011-CXTD-13)；保定市科技攻关资助项目( 编号：08ZF088) |
|
| 摘要点击次数: 1304 |
| 全文下载次数: 1088 |
| 中文摘要: |
| 目的：优选玄参中哈巴苷和哈巴俄苷的提取工艺。方法：采用星点设计-效应面法，以哈巴苷与哈巴俄苷总提取率为指标，考察提取溶剂、液料比和提取时间对其的影响。结果：18%~32%乙醇，液料比20∶1~26∶1，提取30~48min时玄参总提取率最高。结论：星点设计-效应面法优化了玄参中哈巴苷与哈巴俄苷的提取工艺，预测性良好且简便可行。 |
| English Summary: |
| To optimize the extraction process for radix scrophulariae by central composite design and response surface methodology.Methods:Central composite design and response surface methodology was used to optimize the extracting process.The main influential factors of extraction efficiency are ethanol concentration，solvent ratio and reflux time.The conditions of the extraction were evaluated by the total content of harpagide and harpagoside.Results:Analysis indicated the optimum conditions of harpagide and harpagoside are 18%~32% ethanol，20~26 fold solvent，ultrasonic extraction 30~48 minutes.Conclusion:It shows that the optimum process for extracting radix scrophulariae is simple，more convenient and with higher precision. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
