世界中医药

作者	单位
阮善明沈敏鹤王益林红郑丽萍李梦婷张恺	1 浙江中医药大学附属第一医院肿瘤中心，杭州，310006； 2 浙江中医药大学，杭州，310053

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中文摘要:

目的：探索中医解毒法协同化疗在结肠癌辅助治疗阶段的优化治疗模式，并初步探讨作用机制。方法：1）皮下接种CT-26细胞于50只BALB/c小鼠建立移植瘤模型，分为5组：提前干预组10只、延后干预组10只、单纯中药组10只、单纯化疗组10只、模型对照组10只。同时增设阴性对照组10只。2）5组移植瘤模型小鼠以及1组阴性对照组小鼠，治疗期间定期监测体重及瘤体变化。分组取血，分离血清，取瘤称瘤重，计算抑瘤率。3）ELISA法检测各组血清CEA、sCD44含量。结果：1）抑瘤率：两药合用组的抑瘤率约50%~60%，单纯化疗组约40%，单纯中药组约20%。抑瘤率从高到低依次为提前干预组、延后干预组、单纯化疗组、单纯中药组。2）小鼠去瘤体重变化：治疗末提前干预组、单纯中药组小鼠去瘤体重较治疗前增加，单纯化疗组、延后干预组、模型对照组治疗后体重下降。3）ELISA结果显示：各治疗组CEA、sCD44含量较模型对照组均有下降，两药合用组较单药治疗组下降明显。提前干预组、延后干预组、单纯化疗组的CEA、sCD44含量与模型对照组比较差异有统计学意义（P<005）。结论：中医解毒法协同化疗能抑制肿瘤的作用机制可能与降低血清CEA、sCD44表达有关。

English Summary:

To investigate the optimum combination of Disintoxication and chemotherapy in auxiliary treatment phase on colon cancer xenografts in mice, elucidate the potential mechanism of Chinese herbal for adjuvant chemotherapy.Methods:1) Colon cancer cell line CT 26 was injected subcutaneously on 50 BALB/c mice to establish colon cancer xenografts model, they were randomly divided into 5 groups: 10 BALB/C mice in pre intervention group、10 in post intervention group、10 in Herbal only Herbal group、10 in Chemotherapy only group、10 BALB/C mice in model control, the other 10 mice were used as negative control group. 2) Body weight and tumor volume were measured during the treatment in the BALB/c mice from each groups and negative control group. Harvesting blood from different groups, separating serum, weighting tumor weight and calculating anti tumor rate. 3) Detecting content of CEA、sCD44 in serum from different groups by ELISA.Results:1) Tumor inhibition rate: The anti tumor rate was 50% 60% in optimum combination groups, while 40% in chemotherapy group and 20% in Herbal only group. in sequence of Pre intervention group > Post intervention group > Chemotherapy only group > Herbal only group. 2) Change of mice weight without tumor weight: Compared with the weight before the treatment，mice weight increased after treatment in Pre intervention group and Herbal only group. While the result is opposite in chemotherapy group、post intervention group and model control group. 3) Serum level of CEA and sCD44:Compared with model control group, the content of CEA、sCD44 decreased in treatment groups, while with dramatic decline in two therapy combination group than single drug treatment group. Compared with model control group, the content of CEA、sCD44 in pre intervention group、post intervention group and chemotherapy group had statistical significance (P<005).Conclusion:The mechanism of the combination of detoxicating therapy and chemotherapy inhibits tumor perhaps related to decreasing expression of CEA、sCD44.

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