| 引用本文:赵雪松 陈志刚 徐枝芳 高 芳.早期使用三七总皂苷对大鼠脑出血炎性反应的影响[J].世界中医药,2013,8(07):. |
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| 早期使用三七总皂苷对大鼠脑出血炎性反应的影响 |
| The Effect of Early Use of Panax Notoginseng Saponins on Inflammation in Rats with Cerebral Hemorrhage |
| 投稿时间：2013-04-09 |
| DOI：10.3969/j.issn.1673-7202.2013.06.027 |
| 中文关键词: 脑出血 脑组织病理 细胞间黏附分子-1 肿瘤坏死因子-α 三七总皂苷 |
| English Keywords:Cerebral hemorrhage Brain pathomorphology ICAM-1 TNF-α Panax notoginseng saponins |
| 基金项目:高等学校博士学科点专项科研基金(新教师类)(编号:2011001312001);北京中医药大学科研创新团队项目中医脑病形神一体化防治研究团队(编号:2011-CXTD-23) |
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| 中文摘要: |
| 目的:早期使用三七总皂苷对脑出血大鼠(Intracerebral Hemorrhage，ICH)脑组织病理形态和炎性反应递质细胞间黏附分子-1(Intercellular Adhesion Molecule-1，ICAM-1)和肿瘤坏死因子-α(Tumor Necrosis Factor-α，TNF-α)的影响。方法:将SD大鼠随机分为假手术组、模型组和三七总皂苷组，采用胶原酶注射尾状核法制作脑出血模型，观察脑组织病理形态变化;双抗体夹心-酶联免疫吸附实验(Avidinbiotincomplex-ELISA，ABC-ELISA)观察ICH大鼠血清炎性反应递质ICAM-1和TNF-α的变化。结果:HE染色观察到炎性细胞浸润，也证实了脑出血急性期炎性反应反应较剧烈，三七总皂苷显著减轻周围脑组织水肿和神经纤维排列紊乱现象;造模7 d时，模型组血清中的ICAM-1和TNF-α含量增加，三七总皂苷用药7 d可下调ICAM-1(P<0.05)和TNF-α。结论:三七总皂苷对脑出血后脑组织病理形态恢复正常具有促进作用，抑制ICAM-1和TNF-α的释放，可能是神经元保护的作用机制之一。 |
| English Summary: |
| To study the effect of early use of Panax notoginseng saponins (PNS) on the pathomorphological brain tisssues, ICAM-1and TNF-α in rats with cerebral hemorrhage(ICH). Methods: SD rats were randomly divided into sham group, model group and PNS group, and ICH models were made with collagenase Ⅶ injected into the rats’ caudate nucleus. The morphological changes in the brain were observed. ICAM-1 and TNF-α in the serum were measured by the method of enzyme linked immunosorbent assay (ELISA). Results: The region around the hematoma infiltrated by inflammatory cells was observed by HS staining, and PNS significantly reduced the brain edema andameliorated disorderly arrangement of nerve fibers; On the 7th day after ICH, the level of ICAM-1 and TNF-α in the serum was still higher in the model group compared with that in the sham group, and the administration of Panax notoginseng saponins down-regulated ICAM-1(P<0.05) and TNF- α. Conclusion: Panax notoginseng saponins can alleviate the pathomorphological brain tissues of cerebral hemorrhage, and it could inhibit the release of ICAM-1 and TNF- α so as to protect the neurons. |
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