世界中医药

目的：观察清脑滴丸对大鼠急性脑缺血再灌注脑梗死面积，皮层肿瘤坏死因子 α(TNF α)、白细胞介素 1β(IL 1β)、白细胞介素 10(IL 10)改变，探讨淸脑滴丸治疗急性脑梗死的可能机制。方法：线栓法制备大鼠大脑中动脉缺血再灌注模型（MCAO），随机分为正常组，假手术组，模型缺血1.5 h分别再灌注6 h、24 h、48 h、72 h、7 d，清脑滴丸各相应时间点治疗组。TTC染色脑片计算梗死面积。酶联免疫法检测皮层IL 1β、TNF α和IL 10。结果：脑组织TTC染色显示模型各组均出现明显梗死灶，在再灌24～72 h梗死面积最大（P<0.01），清脑滴丸治疗组的梗死面积明显缩减，再灌24~72 h梗死面积减小最为明显（P<0.01）。与假手术组比较，再灌注6 h时IL 1β、TNF α、IL 10出现升高，再灌注48～72 h达高峰（P<0.01），至再灌注7 d仍有明显升高。与各模型组比较，清脑滴丸治疗组明显下调IL 1β和TNF α(P<0.01)；且能明显上调IL 10(P<0.05~0.01)。结论：清脑滴丸能够显著下调脑缺血再灌注后TNF α、IL 1β，上调IL 10，缩小脑梗死面积，减轻脑损伤，提示清脑滴丸治疗急性脑梗死机制可能与调节炎症因子，促炎因子与抗炎因子达到动态平衡有关。

English Summary:

To observe the cerebral infarct area and contents of tumor Necrosis Factor α(TNF α), interleukin 1β(IL 1β) and interleukin 10(IL 10) in rats with acute cerebral infarction, and discuss the mechanism of Qingnao dripping pills in treating such diseases. Methods:The rat models of focal cerebral ischemia and reperfusion were made by thread occlusion technique induced middle cerebral artery occlusion. Rats were randomly divided into 12 groups: control group, sham group, 3 h, 6 h, 12 h, 24 h, 48 h, 72 h reperfusion respectively after 1.5 h ischemia by MCAO, and Qingnao dripping pills groups. Using TTC staining brain to measure infarction area, ELISA to measure IL 1β, TNF α and IL 10. Results:TTC staining display model group had obvious infarcts, and the infarction area was the largest during 24 h to 72 h reperfusion(P<0.01); while after giving Qingnao dripping pills, the infarction area was significantly reduced(P<0.01). Compared with sham group, IL 1β, TNF α and IL 10 appeared to rise at 6 h, and at 48 h and 72 h reached reperfusion peak (P<0.01). There was an increasing rise at 7d reperfusion. Compared with the model groups, IL 1β and TNF α in the Qingnao dripping pills treatment group significantly decreased(P<0.01) and IL 10 obviously increased(P<0.05 ~ 0.01). Conclusion:Qingnao dripping pills can significantly decrease TNF a and IL 1β and increase IL 10; thus reducing cerebral infarction area and improve brain damage. This may due to that it can modulate inflammatory factor, pro inflammatory factor and anti inflammatory factor to achieve dynamic balance.

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