| 引用本文:李言1，梁俊清2，张高峰1，李佳1，杨锡兰1，陈前芬1，赵士弟1.基于络病学探讨内毒素“二次打击”急性肺损伤大鼠的Rho/ROCK机制及川芎嗪的保护作用[J].世界中医药,2016,(01):. |
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| 基于络病学探讨内毒素“二次打击”急性肺损伤大鼠的Rho/ROCK机制及川芎嗪的保护作用 |
| A Modified Lipolysaccharide “Two hit” Induced Acute Lung Injury Model in Rats via Rho/ROCK Mechanism and Protective Effects of Tetramethylpyrazine on Injury Based on Collateral Disease Theory |
| 投稿时间：2015-07-23 |
| DOI：10.3969/j.issn.1673-7202.2016.01.033 |
| 中文关键词: 络病学 内毒素血症 急性肺损伤 川芎嗪 Rho/ROCK |
| English Keywords:Collateral Disease Theory Endotoxemia Acute lung injury (ALI) Tetramethylpyrazine (TMP) Rho/ROCK |
| 基金项目:国家自然科学基金项目（编号：81202833）；安徽省自然科学基金项目（编号：1308085MH140） |
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| 中文摘要: |
| 目的：研究内毒素血症“瘀滞络脉”急性肺损伤大鼠的Rho/ROCK机制及川芎嗪的保护作用。方法：以内毒素“二次打击”建立大鼠内毒素血症“瘀滞络脉”急性肺损伤模型，分别设正常对照组、模型组、川芎嗪低剂量组、高剂量组，测定呼吸频率、肺泡灌洗液中性粒细胞(PMN)百分率、肺湿/干重比、肺组织髓过氧化物酶(Myeloperoxidase，MPO)活性、ROCK mRNA表达量，并观察、评估肺组织损伤严重程度。结果：与模型组相比，川芎嗪显著降低呼吸频率、PMN百分率、肺湿/干重比、MPO活性、ROCK mRNA表达量，减轻大鼠急性肺损伤病理改变。结论：川芎嗪可能通过抑制Rho/ROCK通路对内毒素血症“瘀滞络脉”大鼠急性肺损伤有较好的保护作用。 |
| English Summary: |
| To investigate the protective effect of tetramethylpyrazine (TMP) on acute lung injury (ALI) in endotoxemia rats with “Collaterals Damaged by Toxic Stasis” (YuZhiLuoMai) and its mechanism of Rho/ROCK. Methods:Endotoxemia rats model in ALI with YuZhiLuoMai were established through lipolysaccharide “two hit”. Rats were randomized into 4 groups: G1 (normal control group), G2 (“two hit” group), G3 (low dosage TMP group) and G4 (high dosage TMP group). Measure the respiratory rate, lung wet/dry weight ratio, BALF PMN percentage, pulmonary MPO activity, ROCK2 mRNA expression quantity, observe and evaluate the severity of lung tissue injury. Results:Compared with the G2 rats, TMP significantly reduced respiratory rate, PMN percentage and lung wet/dry weight ratio, MPO activity and ROCK mRNA expression quantity, and reduced the pathological changes in endotoxemia rats with ALI.Conclusion:We found TMP might have better protection against ALI in endotoxemia rats with YuZhiLuoMai through inhibiting RhoA/ROCK pathway. |
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