世界中医药

目的：观察艾灸对ApoE-/-小鼠主动脉内肿瘤坏死因子（TNF-α）、白细胞介素-10(IL-10)及基质金属蛋白酶9（MMP-9）的影响，并从抑制炎性反应，稳定动脉粥样硬化斑块等角度探讨艾灸在防治动脉粥样硬化中的机制。方法：将48只采用高脂饮食喂养的载脂蛋白E基因敲除小鼠（ApoE-/-小鼠）作为动脉粥样硬化模型，并随机分为3组：艾灸组，模型组，药物对照组。并将16只同龄相同遗传背景的C57BL/6小鼠作为空白对照组。空白组、模型组小鼠每天常规抓取、固定，并放置假艾灸装置。艾灸组小鼠每天抓取固定，并艾灸膻中穴。药物组小鼠每天采用辛伐他汀0.28 mg/100 g灌胃，所有干预20 min/d，6 d/周，干预14周后牺牲，取材检测。Elisa法测定主动脉内TNF-α、IL-10、MMP-9的含量。油红“O”染色观察胸主动脉病理改变。结果：1)与模型组相比，艾灸组，药物组小鼠主动脉内TNF-α、MMP-9含量明显降低(P<0.05)，艾灸组，药物组之间无明显差异；与模型组相比，药物组IL-10水平明显升高有统计学意义(P<0.05)，艾灸组小鼠主动脉内IL-10呈升高趋势，但无统计学意义。2)胸主动脉病理改变：与空白组相比，模型组胸主动脉可见明显的AS斑块，内膜破坏，中膜增厚，平滑肌细胞破坏变性，管腔变狭窄。相比于模型组，艾灸组及药物组主动脉病变明显减轻。结论：1)艾灸可以有效缓解动脉粥样硬化病变，抑制动脉粥样硬化斑块生长；2)艾灸可以抑制体内炎性反应，并具有稳定斑块的作用

English Summary:

To observe the effect of moxibustion on TNF-α, IL-10 and MMP-9 of arteriosclerosis mice and discuss its possible mechanism from the angle of inhibiting inflammatory factors and stabilizing plague. Methods: To establish atherosclerosis model by removing ApoE gene through high-fat diet feeding. A total of forty-eight ApoE-/-mice (8 weeks old) were randomly divided into moxibustion group, model group, and medicine control group. Additionally, sixteen same age C57BL/6 mice were used as a blank control. Mice in the blank control and model control group were handled and intervened with sham moxibustion every day. Mice in the moxibustion group were handled and treated with moxibustion on Dan Zhong (RN17) acu-point while the mice in medicine control group were intervened by gavage Simvastatin 0.28 mg/100 g. All the interventions were given 20 min/day, 6 days/week, 14 weeks totally. Then the mice were killed for examination. Elisa method was used to test the level of TNF-α, IL-10, and MMP-9 and red oil “O” stain were used to observe the plague size in the aorta. Results: 1)Compared with the model group, TNF-α, MMP-9 levels of moxibustion group and medicine control group were decreased significantly (P<0.05), and there is no significant difference between the moxibustion group and medicine control group. Compared with the model group, IL-10 levels were increased significantly (P<0.05) in medicine control group. Despite IL-10 levels in moxibustion group showed a trend of increase, no significant difference was shown. 2)The pathological changes in the thoracic aorta plaque: Compared with the blank group, plague in model group can be obviously seen in the thoracic aorta, pathological changes including intimal destruction, medium thickness, smooth muscle cell degeneration, lumen stenosis can also be seen in the model group. Compared with model group, aortic lesions in moxibustion group and medicine group were significantly reduced. Conclusion: 1)Moxibustion could alleviate atherosclerosis, inhibit the growth of plague. 2)Moxibustion could inhibit inflammation in the body, which may be one of the mechanisms of its anti-atherosclerosis effect. Besides, moxibusion also contributes to plaque stability.

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