| 引用本文:刘耀萌，刘钧天，黄畅，韩丽，赵百孝.不同艾灸因素对阿尔茨海默小鼠PI3K/AKT通路与皮质β淀粉样蛋白沉淀的影响[J].世界中医药,2016,(08):. |
|
| 不同艾灸因素对阿尔茨海默小鼠PI3K/AKT通路与皮质β淀粉样蛋白沉淀的影响 |
| Effect of Different Factors of Moxibustion on PI3K/AKT Signal Pathway and Cortical β-amyloid Protein Precipitation in Brain Tissue of Alzheimer Mice |
| 投稿时间：2016-06-28 |
| DOI：10.3969/j.issn.1673-7202.2016.08.002 |
| 中文关键词: 阿尔茨海默病 艾灸 艾烟 无烟灸 PI3K/AKT通路 胰岛素降解酶 |
| English Keywords:Alzheimer′s disease Moxibustion Moxa smoke Heat-treatment moxibustion PI3K/AKT pathway IDE |
| 基金项目:国家自然科学基金项目（编号：81373730）；国家青年自然基金项目（编号：81403449）；国家自然科学基金项目（编号：81574068）；国家国际科技合作专项项目（编号：2011DFA31370） |
|
| 摘要点击次数: 729 |
| 全文下载次数: 886 |
| 中文摘要: |
| 目的：观察艾灸、艾烟、无烟灸干预对APP/PS1小鼠大脑皮质PI3K/AKT通路相关指标和β淀粉样蛋白沉淀的影响，从能量代谢的角度探索艾灸不同作用因素在防治阿尔茨海默病中的机制。方法：将32只5个月龄雄性APP/PS1阿茨海默病模型小鼠随机分为模型组、艾灸组、艾烟组、无烟灸组，每组各8只，并将8只同月龄雄性C57BL/6小鼠作为空白对照组。空白组、模型组小鼠每日常规抓取、固定于正常环境；艾灸组与无烟灸组小鼠每日抓取、固定后取关元穴灸治；艾烟组小鼠每日抓取、固定后暴露于10～15 mg/m3艾烟环境。所有干预20 min/d，6 d/周，共8周。于第10周取材。ELISA法检测各组小鼠大脑皮质中IRS-1、PI3K、AKT、GSK-3α、IDE的含量，刚果红染色检测大脑皮质区β淀粉样蛋白沉淀情况。结果：皮质中IRS-1含量比较显示，模型组低于空白组（P<0.01），艾灸组、艾烟组高于模型组（P<0.01，P<0.05）；脑中PI3K含量比较显示，模型组低于空白组（P<0.01），艾灸组、艾烟组高于模型组（P<0.05）；脑中AKT含量比较显示，模型组低于空白组（P<0.05），艾灸组、艾烟组、无烟灸组高于模型组（P<0.05），艾灸组高于无烟灸组（P<0.05）；脑中GSK-3α含量比较显示，模型组高于空白组（P<0.01），艾灸组、艾烟组、无烟灸组低于模型组（P<0.01，P<0.05，P<0.05）。模型组小鼠在皮质内可观察到明显的橘红色Aβ沉积，空白对照组明显少于模型组(P<0.01)。艾烟组、艾烟组、无烟灸组在相同皮质区域中，淀粉样蛋白沉淀均有不同程度的减少。结论：艾灸与艾烟可以调整APP/PS1阿尔茨海默病小鼠的脑部能量代谢，此外艾烟还可以增加对β淀粉蛋白的清除，减缓小鼠皮质内β淀粉蛋白沉淀的产生速度，以延缓AD病理进程。 |
| English Summary: |
| To observe the effect of smoking moxibustion, none-smoking/ heat moxibustion and moxa smoke on PI3K/AKT signal pathway and cortical β-amyloid protein precipitation and discuss the mechanism of them in preventing and treating Alzheimer. Methods: Eight wide-type C57BL/6 normal mice served as normal control group, and 32 APP/PS1 Alzhermer model mice were randomly divided into four groups (n=8/group): 1) No-treatment control group, 2) Moxibustion group, 3) Moxa smoke group, 4) one-smoking moxibustion/heat group. The treatment were given 20 mins a day, 6 days a week for 8 weeks. In group 2 and 3, the moxa stick was pointed above acupoint Guanyuan(CV4). In group 3, mice were exposed to moxa smoke (10～15 mg/m3). However in group 1 and normal control group, no treatment was given. ELISA technique was used to detect the level of IRS-1, PI3K, AKT, GSK-3α, and IDE. In addition, β-amyloid was evaluated using Congo red staining.Results: Compared to group 1, mice in group 2, group 3 and group 4 had significant higher levels of IRS-1, PI3K, AKT and lower levels of GSK-3α (P<0.05). Comparing with group 1, mice in group 4 had significant higher levels of IDE (P<0.05). Mice in group 1 expressed great aggregation of β-amyloid while mice in normal control group had none aggregation of β-amyloid (P<0.05). Mice in group 2, group 3, group 4 had less aggregation of β-amyloid (P<0.05). Conclusion: Moxibustion, moxa smoke and heat-treatment moxibustion play equal role of delaying the pathological process Alzheimer′s disease by PI3K/AKT pathway and can induce less aggregation of β-amyloid. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
