世界中医药

目的：观察艾灸对动脉硬化小鼠凝血-纤溶系统的调控效应；方法：实验分为正常组（C57BL/6小鼠）、模型组、艾灸膻中组、氯吡格雷干预组（阳性组），其中模型组、艾灸组及氯吡格雷组均选用动脉硬化模型ApoE-/-小鼠，每组15只小鼠，高脂喂养；正常组选用C57BL/6小鼠作为对照，普通饮食喂养。正常组和模型组小鼠釆用固定器固定小鼠头、尾、四肢部位，不予任何处理，每日抓取固定20 min；艾灸膻中组小鼠釆用固定器固定小鼠头、尾、四肢部位后，固定器底板处设有小孔,可暴露胸部膻中穴,点燃特制艾条放置小鼠腹部下方,使灸火透过固定器底部小孔传至小鼠膻中穴。用特制艾条灸膻中20 min；阳性药组小鼠：口服给予氯吡格雷溶液14 mg/kg（1次/d）。各组干预6 d/周，16周后牺牲动物，心脏取血，用Elisa法检测血浆中纤溶系统标志因子t-PA、PAI-1；凝血系统的标志因子TXB2、6-Keto-PGF1a及参与血小板活化过程的重要黏附因子vWF的表达；结果：与模型组相比，艾灸提高血浆中t-PA含量，但无统计学意义，P>0.05；与模型组相比，艾灸显著性降低血浆内PAI-1的含量，P<0.05；与模型组相比，艾灸显著性降低黏附因子VWF的含量，P<0.05；与模型组相比，艾灸显著性提高血浆中6-Keto-PGF1α的含量，P<0.05；与模型组相比，艾灸增加血浆中TXB2含量，但无统计学意义，P>0.05。结论：艾灸对凝血-纤溶系统有良好的调节作用，可以通过下调PAI-1的含量，提高纤溶系统的活性，上调6-Keto-PGF1α/TXB2的比值，降低黏附因子vWF，进一步发挥其调控血小板活化，防治动脉硬化的效应。

English Summary:

To observe the regulating effect of moxibustion on fibrinolytic clotting factors in apolipoprotein E-deficient (ApoE-/-) arteriosclerosis (AS) mice. Methods: ApoE-/- AS mice were randomly divided in to model group, moxibustion group and clopidogrel group, with 15 mice in each, and fed with high fat. In addtion, 15 C57BL/6 mice were chosen as the normal control group, fed with normal diet. The normal control group and model group were both only given fixation to mice without any treatment, 20 min/day. Moxibustion group was given moxibustion on Dan Zhong (CV17) acu-point 20 min/day. Besides, the clopidogrel group was given clopidogrel 14 mg/kg. After 16 weeks of intervention (6 days/week), mice were killed to take the heart blood as to test the level of t-PA and PIA-1 using ELISA method. Also, the expression of TXB2，6-Keto-PGF1a and vWF were tested. Results: Comparing with the model group, t-PA and TXB2 in the moxibusiton group was enhanced with no statistical significance (P>0.05), while the PAI-1 and vWF were decreased significantly (P<0.05). Conclusion: Moxibustion can regulate fibrinolytic clotting factors positively by down-regulating PAI-1 and activate fibrinolytic system, up-regulating 6-Keto-PGF1α/ TXB2, decreasing vWF thus activating thrombocyte and preventing AS.

查看全文查看/发表评论下载PDF阅读器