| 引用本文:孙宝莹1,2，郭涛2，李西文1，陈士林1，孙奕1.葛根饮片标准汤剂的研究[J].世界中医药,2016,(08):. |
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| 葛根饮片标准汤剂的研究 |
| Preparation and Quality Standard Study on the Kudzuvine Root Standard Decoction |
| 投稿时间：2016-04-12 |
| DOI：10.3969/j.issn.1673-7202.2016.08.053 |
| 中文关键词: 葛根 标准汤剂 质量标准 指纹图谱 |
| English Keywords:Kudzuvine Root Quality standard Standard decoction Fingerprint |
| 基金项目:中国中医科学院综合性大平台项目（编号：Z2014003） |
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| 中文摘要: |
| 目的：制备葛根饮片标准汤剂，并进行质量标准研究。方法：药材经DNA条形码基原鉴定，均为豆科植物葛根Pueraria lobata（willd.）Ohwi的干燥根。采用水煎煮的方法制备葛根饮片的标准汤剂，并考察葛根标准汤剂中葛根素含量测定的色谱条件，对不同地区的葛根水煎液进行指纹图谱测定，并分别计算葛根素的转移率和葛根饮片标准汤剂的出膏率。结果：通过对10批不同产地葛根的标准汤剂及其饮片样品进行测定，葛根素转移率范围为54.3%～79.1%，出膏率范围为19.0%～38.5%；同时对10批葛根饮片制作出相应的指纹图谱，标定了5个共有峰，计算出其相似度在94%以上。结论：10批次不同产地的葛根标准汤剂与葛根饮片的基本属性相一致，建立本研究的葛根标准汤剂制备方法规范，质量符合标准要求。 |
| English Summary: |
| To prepare standard decoction and to study the quality standards of Kudzuvine Root. Methods: The medicinal medica was evaluated by DNA bar code to make sure the medica was Kudzuvine Root′s dry root. The The method of standard decoction preparation of Kudzuvine Root and its determination of chromatogram conditions were adopted so as to determine the fingerprint of standard decoction and calculate the transfer rate and the extractum rate of the standard decoction. Results: After the measurement of standard decoction of 10 batches of Kudzuvine Root from different regions and sample pieces, the transfer rate ranged from 54.3% to 79.1% and extractum rate was at the range of 19.0%~38.5%; Moreover, the 10 batches of Kudzuvine Root produced the corresponding fingerprint, 5 common peaks were calibrated, and the similarity was more than 94%. Conclusion: The basic properties of 10 batches of Kudzuvine Root Decoction are consistent with its prepared herbal medicine standard. Therefore, it can be used to establish the standard decoction of Kudzuvine Root. |
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