| 引用本文:吴彦青1，郭玉红1，2，3，刘清泉1，2，3.C57BL/6J小鼠实验性自身免疫性脑脊髓炎模型建立的关键性问题[J].世界中医药,2017,(04):. |
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| C57BL/6J小鼠实验性自身免疫性脑脊髓炎模型建立的关键性问题 |
| Key issues on establishment of the experimental autoimmune encephalomyelitis model in C57BL/6J mice |
| 投稿时间：2017-03-17 |
| DOI：10.3969/j.issn.1673-7202.2017.04.006 |
| 中文关键词: 实验性免疫性脑脊髓炎模型 关键性问题 免疫前处理 免疫操作 免疫后护理 |
| English Keywords:Experimental autoimmune encephalomyelitis Key issues Preparation before immunization Operation in immunization Nursing after immunization |
| 基金项目:国家自然科学基金项目(编号:81403221) |
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| 中文摘要: |
| 实验性自身免疫性脑脊髓炎(EAE)模型是国际公认的多发性硬化(MS)的经典动物模型，被广泛用于MS发病机制和评价免疫调节药物的实验研究。实验操作中仍有较多缺陷导致发病率不能达到100%和死亡率偏高，需要对造模的关键技术不断完善。加强该模型免疫前准备工作、免疫操作和免疫后护理3个方面的研究，分析EAE模型成功建立的关键性问题，并根据实验操作过程提出解决办法，完善EAE模型的规范化造模流程，可以使EAE模型具备发病率高、死亡率低、模型稳定的特点。 |
| English Summary: |
| Experimental autoimmune encephalomyelitis (EAE) model is an internationally recognized classic animal model of multiple sclerosis (MS) and is widely used in researches on the pathogenesis of MS and the evaluation of immunomodulator for MS. However， There are still many defects leading to morbidity less than 100% and high mortality. Therefore， in order to reach a high morbidity， a low mortality and a stable EAE model， the process of EAE model establishment was standardized and refined by improving the preparation， operation and nursing of the model immunization. In addition， the key issues to the successful establishment were analyzed and the solutions were provided according to the process of experiment operation. |
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