世界中医药

作者	单位
杨蕾，王继峰，牛建昭，赵丕文，孙丽萍，陶仕英，武洪波，蔡欣悦，齐明月	北京中医药大学，北京，100029

摘要点击次数: 1420

全文下载次数: 1246

中文摘要:

目的：观察二仙汤含药血清对顺铂干预的大鼠卵巢颗粒细胞凋亡调控蛋白bcl-2、bax表达的影响，从细胞凋亡的角度探讨二仙汤含药血清对卵巢颗粒细胞凋亡的作用机制。方法：22～24 d的SD雌性大鼠30只，随机分为3组，分别为生理盐水组，补佳乐组，二仙汤组。每组大鼠10只。给药4 d后处死大鼠，心脏取血，制备药物血清。22～24 d的SD大鼠卵巢颗粒细胞，经培养贴壁后，用顺铂损伤大鼠原代卵巢颗粒细胞。经各组药理血清作用后，应用放免法，进行雌激素和孕激素水平测定及分析。应用免疫组化法，测定各分组bcl-2、bax蛋白的表达情况，并进行图像分析。结果：放免结果显示，与正常组相比，模型组和补佳乐组E2表达明显改变，有统计学意义(P<0.05)。与模型组相比，补佳乐组、二仙汤组E2表达均有增加；与正常组相比，模型组P表达水平有所变化，有统计学意义(P<0.05)。与模型组相比，二仙汤组P表达略高于补佳乐组，但低于正常组。免疫组化结果显示，在卵巢颗粒细胞细胞质上可见bcl-2和bax蛋白表达的棕黄色颗粒。和正常组相比较，顺铂干预后各组bcl-2表达均减弱，而以模型组最明显，有统计学意义(P<0.05)；和模型组比较，各药物组均可见bcl-2阳性表达增加，但弱于正常组，有统计学意义(P<0.05)。bax与bcl-2表达强度结果相反，和正常组比较，顺铂干预后的各组bax均表达增强，模型组呈现极强表达现象，有统计学意义(P<0.05)；和模型组比较，各药物组均可见阳性表达减弱，但表达高于正常组，有统计学意义(P<0.05)。结论:二仙汤能降低经顺铂干预后大鼠bax蛋白在卵泡颗粒细胞表达，增强bcl-2蛋白的表达，二仙汤能够通过调节凋亡蛋白bcl-2、bax蛋白的表达，抑制卵巢颗粒细胞发生凋亡，从而保护卵巢功能。

English Summary:

To observe the effect of serum containing Erxian decoction on regulating the expression of bcl-2and bax protein from rats’ ovarian granulosa cells induced by cisplatin and to investigate the mechanism of Erxian Decoction serum on apoptosis of ovarian granulosa cells from the perspective of apoptosise. Methods:Thirty SD female rats for 22 to 24 days birth were randomly divided into three groups：the normal saline group，progynova group and the Erxian Decoction group with 10 rats in each group. After 4-day drug administration，rat hearts were taken to prepare drug serum. Ovarian granulosa cells were taken from SD rats and cultured to acquire adherent cells. Then，primary ovarian granulosa cells in rats were induced by cisplatin. After pharmacological action to serum，radio immunity method was used to detect estrogen and progestin and make analysis. Immunohistochemistry was applied to detect the expression of bcl-2 and bax protein in each group and then for image analysis. Results:Test results showed that E2 expression significantly changed in the model group and progynova group (P<0.05) with statistically significant difference，compared with the normal group. Compared with the model group，the expression of E2 increased significantly in the progynova group and the Erxian Decoction group. Compared with the normal group，P expression in the model group was somewhat changed (P<0.05)，which showing statistically significant difference. Compared with the model group，P expression in Erxian Decoction group was slightly higher than that of the progynova group，but lower than that of the normal group. Immunohistochemistry results showed that brown granules of bcl-2 and bax protein were expressed in the cytoplasm of ovarian granulosa cells. Compared with the normal group，the expression of bcl-2 in each group under the intervention of cisplatin was decreased，and the model group was the most obvious (P<0.05).Compared with model group，the positive expression of bcl-2 in each drug group was increased，but it was weaker than that in the normal group (P<0.05). The intensity of bax and bcl-2’s expression was opposite. Compared with the normal group，Bax expression in each group under the intervention of cisplatin were enhanced and that in the model group especially showed a strong expression (P<0.05). Compared with the model group，the positive expression of the drug groups was decreased，but was higher than that of the normal group (P<0.05) with statistically significant difference. Conclusion:Erxian Decoction can decrease the expression of Bax protein in follicular granulosa cells induced by cisplatin injury and increase the expression of bcl-2 protein. It can inhibit the apoptosis of ovarian granulosa cells and protect the ovarian function by adjusting the expression of bcl-2 and bax.

查看全文查看/发表评论下载PDF阅读器