世界中医药
文章摘要
引用本文:萨茹拉1,2,吴玉小2,吴伟红2,白珍3,白长喜3.蒙药熊胆治疗应激性胃溃疡出血模型大鼠的主要成分及机制研究[J].世界中医药,2018,(01):.  
蒙药熊胆治疗应激性胃溃疡出血模型大鼠的主要成分及机制研究
Experimental Study on the Main Components and Mechanisms of Mongolian Medicine Bear Bile to Stress Gastric Ulcer Hemorrhage Model Rats
投稿时间:2017-02-05  
DOI:10.3969/j.issn.1673-7202.2018.01.039
中文关键词:  蒙药熊胆  主要成分  胃溃疡出血  作用机制
English Keywords:Mongolian bear bile  Main components  Gastric ulcer bleeding  Action mechanism
基金项目:国家自然科学基金项目(81541171);内蒙古自治区科技计划项目资助(kjt15sf19);内蒙古自治区蒙药重点实验室新药创新项目
作者单位
萨茹拉1,2,吴玉小2,吴伟红2,白珍3,白长喜3 1 天津中医药大学天津300193 2 内蒙古自治区国际蒙医医院呼和浩特010065 3 内蒙古医科大学蒙医药研究所呼和浩特010020 
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中文摘要:
      目的:探讨蒙药熊胆治疗应激性胃溃疡出血模型大鼠的主要成分及作用机制。方法:将130只SD大鼠随机分为13组,即空白组、模型组、奥美拉唑组、蒙药止血红花-8味散低和高剂量组、熊胆粉低和高剂量组、熊胆主要成分即熊去氧胆酸(UDCA)低和高剂量组、牛磺熊去氧胆酸(TUDCA)低和高剂量组、胆酸(CA)低和高剂量组等,每组10只,大鼠按分组依次给药实施治疗,观察及分析用药后对血小板计数、大鼠血清5-羟色胺(5-HT)、血管紧张素Ⅱ(AngⅡ)和血管内皮生长因子(VEGF)等指标的影响及病理变化。结果:与空白组比较,模型组大鼠血小板计数降低、血清5-HT、AngⅡ的含量及VEGF含量明显升高,差异比较,均有统计学意义(P<0.05);与模型组比较,空白组、奥美拉唑阳性对照组、蒙药熊胆及主要成分观察组血小板计数明显升高,血清5-HT,AngⅡ,VEGF水平明显下降趋势,差异均有统计学意义(P<0.05);与奥美拉唑阳性对照组比较,除了CA低和高剂量组外蒙药熊胆及主要成分各组血清5-HT、AngⅡ和VEGF水平变化比较,差异均无统计学意义(P>0.05);UDCA及TUDCA组效果尤为明显。光镜下可见正常组大鼠胃黏膜、胃底腺结构连接完整,模型组有溃疡病变,蒙药熊胆及主要成分观察组均有明显的改善趋势。结论:蒙药熊胆及主要成分UDCA和TUDCA对胃溃疡出血有很好的治疗和防治作用;蒙药熊胆治疗胃溃疡出血的主要成分为UDCA和TUDCA,CA作用相比较弱。
English Summary:
      To investigate the main component and mechanisms of Mongolian Medicine bear bile in the treatment of stress ulcer hemorrhage model rats.Methods:A total of 104 SD rats were randomly divided into 13 groups, namely blank group, model group, Caffeic acid positive group, mongolian Hemostasis Honghua-8 pill low and high dose group, bear bile low and high dose group, UDCA low and high dose group, TUDCA low and high dose group, CA low and high dose group, 10 rats in each group.The rats were treated by sequential administration.The impact of Platelet, 5-hydroxy tryptamine (5-HT), angiotensinⅡ (AngⅡ) and vascular endothelial growth factor (VEGF) and the pathological changes was observed and analyzed.Results:Compared with the blank group, the content of platelet reduced.5-HT, AngⅡ and VEGF were significantly increased, which was statistically significant (P<0.05).Compared with model group, bear bile and the main component of platelet increased in blank group, positive group, Mongolian Medicine treatment group, and 5-HT, AngⅡ and VEGF were showing significantly decreasing tendency (P<0.05).Compared with the Omeprazole positive group, except for CA low and high dose groups, Mongolian bear bile and its main effective component of 5-HT, AngⅡand VEGF were not significantly different (P>0.05).The effects of the UDCA and TUDCA groups were particularly significant.Under light microscope, the structure of gastric mucosa and gastric fundus gland of rats in the blank group were connected, and the ulcer in the model group was significantly improved.Conclusion:Mongolian bear bile and its main components, UDCA and TUDCA, have a good treatment and prevention of gastric ulcer bleeding.The main effective components of Mongolian bear bile in the treatment of gastric ulcer bleeding are UDCA and TUDCA, but the effect of CA was weak.
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