世界中医药
文章摘要
引用本文:张伟贤1,2,兰天1,3,董嘉乐3,郭姣1,2.山柰酚抵抗对乙酰氨基酚引起的肝细胞损伤研究[J].世界中医药,2019,(01):.  
山柰酚抵抗对乙酰氨基酚引起的肝细胞损伤研究
Protective Effects of Kaempferol Against Acetaminophen in Hepatocytes-Induced Injury
投稿时间:2018-12-10  
DOI:10.3969/j.issn.1673-7202.2019.01.008
中文关键词:  山柰酚  对乙酰氨基酚  凋亡  肝细胞保护
English Keywords:Kaempferol  Acetaminophen  Apoptosis  Hepatocyte protection
基金项目:广东省科技厅实验室建设项目(2017B030314064);广东省科技厅国际合作基地建设项目(2016B050501003)
作者单位
张伟贤1,2,兰天1,3,董嘉乐3,郭姣1,2 1 广东省代谢病中西医结合研究中心广州510006 2 广东省代谢性疾病中医药防治重点实验室广州510006 3 广东药科大学药学院广州510006 
摘要点击次数: 915
全文下载次数: 843
中文摘要:
      目的:探究山柰酚(KA)抵抗对乙酰氨基酚(APAP)诱导的肝细胞损伤的作用。方法:CCK-8法检测KA对肝细胞活性的影响,检测细胞LDH释放比率确定APAP诱导肝细胞损伤模型的浓度和时间;CCK-8法检测肝细胞活性,检测肝细胞释放的LDH,观察肝细胞形态评估KA对对乙酰氨基酚诱导肝细胞损伤的影响;Western Blot检测p-JNK、JNK、EndoG蛋白表达。结果:本研究采用5 mmol/L APAP诱导肝细胞24 h建立药物肝损伤细胞模型。与APAP组比较,KA组肝细胞细胞的形态明显改善,凋亡细胞明显减少(P<0.01),LDH释放比率明显下降(P<0.001),Western Blot结果表明经KA治疗后,肝p-JNK和Endo G蛋白表达明显下调。结论:该研究表明KA通过抑制p-JNK和Endo G来抵抗APAP诱导肝细胞凋亡以达到肝细胞的保护作用。
English Summary:
      To investigate the protective effects of kaempferol against acetaminophen in hepatocytes-induced injury. Methods:Cholecystokinin-8 (CCK-8) was used to detect the vitality of hepatocytes treated by kaempferol. The lactate dehydrogenase (LDH) released ratio was detected,which could determine the concentration and time of acetaminophen (APAP)-induced hepatocytes injury model. CCK-8 and LDH released ratio were used to detect the vitality of hepatocytes treated by APAP and kaempferol. In the meanwhile,hepatocyte morphology was to be record. P-Jun N-terminal kinase (P-JNK),Jun N-terminal kinase (JNK),Endo G protein expression were detected by Western Blot searching. Results:The APAP concentration was determined to be 5 mmol/L and the time was 24 hours to induce liver injury cell with drug. Compared with the APAP group,the morphology of hepatocytes was significantly improved. The ratio of apoptotic cells decreased significantly (P<0.01),and the ratio of released LDH content decreased significantly (P<0.001). Western Blot showed that P-JNK and Endo G were significantly down-regulated. Conclusion:This experiment demonstrates that kaempferol has a protective effect on APAP-induced hepatocytes injury.
查看全文  查看/发表评论  下载PDF阅读器