| 引用本文:任娟1，2，刘晓2，李伟东2，蔡皓2，朱晓钗2，蔡宝昌2，3，4.基于UHPLC-Q-TOF-MS/MS的白芍炒制前后化学成分研究[J].世界中医药,2019,(02):. |
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| 基于UHPLC-Q-TOF-MS/MS的白芍炒制前后化学成分研究 |
| Qualitative Analysis of Major Constituents of Raw and Processed Paeonia Lactiflora by UHPLC-Q-TOF-MS/MS |
| 投稿时间：2019-01-10 |
| DOI：10.3969/j.issn.1673-7202.2019.02.003 |
| 中文关键词: 白芍 炒制 UHPLC-Q-TOF-MS/MS 四极杆 化学成分 |
| English Keywords:Paeonia lactiflora Processing UHPLC-Q-TOF-MS/MS Quadrupole Chemical components |
| 基金项目:国家自然科学基金面上项目(81873004) |
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| 中文摘要: |
| 目的：采用超高效液相色谱串联四极杆飞行时间质谱法，探讨白芍炒制前后化学成分的变化。方法：采用Agilent C18色谱柱，0.1%甲酸水溶液-乙腈梯度洗脱，体积流量0.3 mL/min，串联四级杆飞行时间质谱分别测定不同产地各10批生、炒白芍中的化学成分。结果：分别在生、炒白芍中鉴定出40种化学成分。结论：通过UHPLC-Q-TOF-MS/MS技术，为分析生、炒白芍中的化学成分提供一种快速、高效的分析方法，为综合评价生白芍和炒白芍的质量提供参考。 |
| English Summary: |
| By using ultra-high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry，to investigate the changes of chemical composition in Paeonia lactiflora before and after progressed.Methods:Agilent C18 column，0.1% formic acid aqueous solution-acetonitrile gradient elution，volume flow rate 0.3 mL/min，tandem four-stage rod time-of-flight mass spectrometry were used to determine the chemical composition of 10 batches of raw and fried white peony in different producing areas.Results:A total of 40 chemical components were identified in raw Paeonia lactiflora (RPL) and processed Paeonia lactiflora (PPL).Conclusion:UHPLC-Q-TOF-MS/MS technology provides a fast and efficient analytical method for the analysis of chemical constituents in RPL and PPL，which provides a reference for comprehensive evaluation of the quality of RPL and PPL. |
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