| 引用本文:朱星宇1，2，3，周燕萍2，3，陆金兰2，3，林上阳2，3，谢辉2，3，夏晨洁2，3，李伟东2，3.杜仲不同炮制品的水提液指纹图谱对比研究[J].世界中医药,2019,(02):. |
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| 杜仲不同炮制品的水提液指纹图谱对比研究 |
| Comparative Study on Fingerprints of Decoctions of Processed Products of Eucommiae Cortex |
| 投稿时间：2019-01-10 |
| DOI：10.3969/j.issn.1673-7202.2019.02.004 |
| 中文关键词: 杜仲；炮制品；HPLC指纹图谱；化学模式识别 水提液 聚类分析 成分分析 |
| English Keywords:Eucommiae Cortex Processed products Fingerprint Chemical pattern recognition Water extract Cluster analysis Component analysis |
| 基金项目:国家自然科学基金面上项目(81773902)——基于“咸入肾”、“肾主骨”理论研究青蛾丸中杜仲、补骨脂盐炙增效机理 |
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| 中文摘要: |
| 目的：建立杜仲不同炮制品（生品、盐品与炭品）的水提液指纹图谱，从整体水平表征杜仲炮制前后的化学成分变化，为杜仲质量控制提供参考。方法：采用HPLC法，Kromasil 100-5-C18色谱柱，0.3%磷酸水溶液-乙腈为流动相，梯度洗脱，流速为1 mL/min，检测波长为238 nm。建立10批杜仲不同炮制品水提液的指纹图谱，利用中药指纹图谱相似度评价系统（2012A版）计算相似度，同时运用聚类分析（HCA）和主成分分析（PCA）对杜仲炮制前后进行模式识别分析。结果：建立了杜仲不同炮制品的水提液指纹图谱，生品标定了7个共有峰，盐品标定了7个共有峰，炭品标定了8个共有峰，并指认了其中5个共有峰。HCA和PCA模式识别能够明显区分杜仲不同炮制品。结论：HPLC指纹图谱结合化学模式识别能够有效地区分杜仲不同炮制品，为其质量控制研究提供参考。 |
| English Summary: |
| To establish the fingerprints of water extracts from different processed products (raw，salt and charcoal) of Eucommiae Cortex to characterize the changes of chemical components before and after processing at the overall level，and to provide reference for the quality control of Eucommiae Cortex.Methods:HPLC method was applied，and Kromasil 100-5-C18 column was used with 0.3% phosphoric acid in water (v/v)-acetonitrile as the elution system in gradient elution mode.The flow rate was 1.0 mL/min and the detective wavelength was 238 nm.Fingerprint Similarity Evaluation System (2012 A edition) was used to establish fingerprints of 10 batches of different processed Eucommiae Cortex products.Meanwhile，Hierarchical Cluster Analysis (HCA) and Principal Component Analysis (PCA) as methods of pattern recognition were applied to give an overall evaluation.Results:The common modes of HPLC fingerprint for different processed Eucommiae Cortex were set up.There were 7 common peaks in crude and salt products，respectively，and 8 common peaks for the charcoal products.Conclusion:The combination of fingerprints and chemical pattern recognition was an effective method for the distinction between different processed Eucommiae Cortex.The results would provide meaning references for quality evaluation of Eucommiae Cortex. |
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