世界中医药

目的：观察麻杏石甘汤对肺炎模型小鼠的疗效，同时探讨其对共刺激分子CD137水平的影响。方法:选取60只无特定病原体（SPF级）幼龄小鼠纳入研究，并随机分为空白组、模型组及中药组，每组20只。其中模型组及中药组小鼠接受肺炎克雷伯菌混悬液滴入鼻腔，空白组小鼠接受等剂量生理盐水滴鼻，24 h中药组小鼠接受麻杏石甘汤灌胃，1剂/d，共干预7 d。比较3组小鼠一般活动情况、外周血中的白细胞计数、多核粒细胞激素（PMN）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、肺组织病理改变以及外周血单核细胞中共刺激分子CD137水平的变化。结果：空白组小鼠未出现任何阳性体征，模型组及中药组小鼠出现精神萎靡、体质量下降、活动减少等相关阳性症状，其中中药组较模型组改善；模型组及中药组小鼠外周血白细胞计数、PMN、IL-6及IL-8均较空白组升高，差异有统计学意义（P<0.05），其中中药组优于对照组（P<0.05）。模型组及中药组小鼠肺组织细胞结构错乱、肺泡大小不一，各级支气管周围、小叶间及肺泡间隔等存在大量炎性细胞浸润，部分有纤维增生。其中中药组较模型组病理明显改善。模型组及中药组小鼠外周血共刺激分子CD137表达增高，与空白组比较差异有统计学意义（P<0.05），其中中药组水平低于模型组，差异有统计学意义（P<0.05）。结论：麻杏石甘汤加减对肺炎有明显临床效果，其作用机制可能与抑制共刺激分子CD137的分泌有关。

English Summary:

To observe the curative effect of Maxing Shigan Decoction on pneumonia model mice，and to explore its effects on the level of costimulatory molecule CD137. Method:A total of 60 SPF-free young mice were randomly divided into blank group，model group and Traditional Chinese Medicine group with 20 mice in each group. The mice in the model group and Traditional Chinese Medicine group were given Klebsiella pneumoniae suspension by dripping into the nasal cavity，the mice in the blank group were given the same dose of normal saline nasal drops，the mice in Traditional Chinese Medicine group were given Maxing Shigan Decoction intragastric administration for 24 hours，one dose per day，for 7 days. The general activity，peripheral blood (WBC)，polymorphonuclear hormone (PMN)，interleukin-6 (IL-6)，interleukin-8 (IL-8)，lung histopathology and the concentration of costimulatory molecule CD137 in peripheral blood mononuclear cells were compared. Results:There were no positive signs in the blank group mice，but the model group mice and Traditional Chinese Medicine group mice showed mental dispirity，weight loss，decreased activity，and decreased fur gloss，and Traditional Chinese Medicine group mice were better than the model group mice. WBC，PMN，IL-6 and IL-8 in the peripheral blood of the model group mice and Traditional Chinese Medicine group mice were higher than the blank group mice，the difference was statistically significant (P<0.05)，Traditional Chinese Medicine group was better than the control group (P<0.05). There were a lot of inflammatory cells infiltrating around bronchi，interlobular and alveolar septum，and some fibrosis in the model group and Traditional Chinese Medicine group. Traditional Chinese Medicine group improved significantly compared with the model group. The expression of costimulatory molecule CD137 in peripheral blood of mice in model group and Traditional Chinese Medicine group was higher than that in blank group (P<0.05). The level of costimulatory molecule CD137 in Traditional Chinese Medicine group was lower than that in model group (P<0.05). Conclusion:Maxing Shigan Decoction has obvious clinical effect on pneumonia，and its mechanism may be related to the inhibition of the secretion of costimulatory molecule CD137.

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