| 引用本文:李雯婷，苗水，陈铭，周恒，兰岚，毛秀红，季申.液相色谱-串联质谱法同时测定丹参中139种农药残留[J].世界中医药,2019,(04):. |
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| 液相色谱-串联质谱法同时测定丹参中139种农药残留 |
| Simultaneous Determination of 139 Pesticide Residues in Salvia miltiorrhiza by LC-MS/MS |
| 投稿时间：2019-03-10 |
| DOI：10.3969/j.issn.1673-7202.2019.04.001 |
| 中文关键词: 液相色谱-串联质谱法 农药残留 QuEChERS法 丹参 分散固相萃取 固相萃取 盐析剂 匀浆法 |
| English Keywords:LC-MS/MS Pesticide residues QuEChERS Salvia miltiorrhiza dSPE SPE Salting-out agent Homogenate |
| 基金项目:广西壮族自治区科学技术厅重大专项计划（2015AC05038）；国家中药标准化项目（ZYBZH-C-GX-09）；国家药典委员会项目（ZG2016-2） |
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| 中文摘要: |
| 目的：采用液相色谱-串联质谱法建立丹参中农药多残留的测定方法。方法：样品经改良的QuEChERS方法提取，采用分散固相萃取(d-SPE)二次净化法净化，并采用液相色谱-串联质谱技术在多反应监测(MRM）模式下测定，内标法定量。结果：丹参中139种农药在0.005～0.2 mg/L范围内线性关系均良好；在10、50和100 μg/kg添加水平下，除了噻菌灵回收率偏低（67.2～67.9）外，其余农药的平均回收率为70.2%～126.1%，相对标准偏差(RSD)为1.2%～19.5%，139种农药的检测限为0.005～0.05 mg/kg。结论：该方法样品前处理简单快速，灵敏度、准确度和精密度均符合农药多残留检测技术的要求，适用于丹参中139种农药残留的快速筛查测定。 |
| English Summary: |
| To study the multi-residues detection methods for Salvia miltiorrhiza by LC-MS/MS.Methods:A method using a modified QuEChERS and d-SPE clean-up followed by LC-MS/MS has been established for the quantitative determination of the residues.The extract was determined by LC-MS/MS in multi-reaction monitoring(MRM）mode，and internal standard method was applied to quantify the pesticides.Results:All the 139 pesticides showed good linearity in the range of 0.005～0.2 mg/L.The average recoveries of the pesticides at the spiked levels of 10，50，100 μg/kg ranged from 70.2～126.1% with the RSDs of 1.2～19.5%，except for Thiabendazole(67.2～67.9%).The limits of detection(LODs）of 139 pesticides were 0.005～0.05 mg/kg.The results demonstrated that this method was simple，rapid and suitable for the determination of 139 pesticide residues in Salvia miltiorrhiza.Conclusion:The analytical sensitivity and accuracy can meet the requirements of multiple pesticide residue analysis. |
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