| 引用本文:冯睿1，夏晶1，潘杰1，2，胡青1，毛秀红1，季申1.不同种类牛黄投料的小儿化毒胶囊（散）中胆红素的研究[J].世界中医药,2019,(04):. |
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| 不同种类牛黄投料的小儿化毒胶囊（散）中胆红素的研究 |
| Study on Bilirubin in Xiaoer Huadu Capsule Made of Different Calculus Bovis |
| 投稿时间：2019-03-10 |
| DOI：10.3969/j.issn.1673-7202.2019.04.002 |
| 中文关键词: 小儿化毒胶囊（散） 人工牛黄 体外培育牛黄 游离胆红素 总胆红素 部分胆红素 高效液相 |
| English Keywords:Xiaoer Huadu Capsule(Powder) Artificial calculus bovis Cultured calculus bovis in vitro Free bilirubin Total bilirubin Portion bilirubin HPLC |
| 基金项目:上海市科学技术委员会基金项目（16DZ0500201，18DZ2292200） |
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| 中文摘要: |
| 目的：建立“小儿化毒胶囊（散）”中游离胆红素、总胆红素以及部分胆红素的HPLC测定方法，比较以人工牛黄、体外培育牛黄2种牛黄分别投料的小儿化毒散之间差异。方法：分别以二氯甲烷、10%草酸溶液（含0.15%十六烷基三甲基氯化铵）-二氯甲烷、0.2 mol/L乙二胺四醋酸二钠-二氯甲烷提取样品中游离胆红素、部分胆红素、总胆红素，用HPLC法测定，固定相为C18色谱柱，流动相为乙腈-1％冰乙酸（95:5），检测波长450 nm，柱温为25 ℃，流速为1.0 mL/min。结果：胆红素在5.64～282 ng范围内线性关系良好，游离胆红素、部分胆红素、总胆红素平均加样回收率为90%～95%，RSD分别为4.2%、4.1%和4.9%，2种牛黄投料的小儿化毒散中胆红素含量及形态存在明显差异。结论：建立的方法可有效识别“小儿化毒胶囊（散）”中牛黄的投料种类，为其他含有牛黄的中药制剂质量评价提供了借鉴。 |
| English Summary: |
| To establish an HPLC methods for free，portion and total bilirubin in Xiaoer Huadu Capsule(Powder）and compare the difference between 2 types of Calculus Bovis.Methods:The free，portion and total bilirubin were extracted with dichloromethane，10% oxalic acid solution(containing 0.15% cetyltrimethylammonium chloride)-dichloromethane，0.2 mol/L disodium ethylenediaminetetraacetate-dichloromethane，determined by HPLC with C18 column，the mobile phase was acetonitrile-1%glacial acetic acid(95:5).The detection wavelength is 450 nm，and the column temperature was 25 ℃.The flow rate was 1.0 mL/min.Results:Bilirubin showed good linear relationship in the range of 5.64 ng-282 ng，and the average recoveries of free，portion and total bilirubin was 90%-95%，with RSD 4.2%，4.1% and 4.9%.There were significant differences in the content and form of bilirubin of 2 kinds of Calculus Bovis.Conclusion:The method established is easy to operate with high effectiveness in identifying bezoar in Xiaoer Huadu Jiaonang and other Chinese traditional patent medicines containing Calculus Bovis. |
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