| 引用本文:张静娴，胡青，董洪霜，孙健，冯睿，于泓，张甦，毛秀红，季申.超高效液相色谱-三重四级杆质谱法用于阿胶糕类食品中阿胶的鉴别及马、牛、羊、猪皮源成分的检测[J].世界中医药,2019,(04):. |
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| 超高效液相色谱-三重四级杆质谱法用于阿胶糕类食品中阿胶的鉴别及马、牛、羊、猪皮源成分的检测 |
| Development of UHPLC-QQQ MS Method for Identification of Donkey-hide Gelatin Cake and Detection of Constituents from Horse，Ox，Sheep or Pig Skin |
| 投稿时间：2019-03-10 |
| DOI：10.3969/j.issn.1673-7202.2019.04.008 |
| 中文关键词: 阿胶 阿胶糕 超高效液相色谱-三重四级杆质谱法 多重反应检测 特征肽 蛋白质组学技术 胶原蛋白 |
| English Keywords:Colla corii asini Donkey-hide gelatin cake UHPLC-QQQ MS MRM Characteristic peptide Proteomics Collagen |
| 基金项目:上海市科学技术委员会基金项目（18DZ2292200） |
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| 中文摘要: |
| 目的：建立以特征肽为检测指标的阿胶糕中阿胶的鉴别方法与马皮、牛皮、羊皮、猪皮源成分的检测方法。方法：采用三氯乙酸沉淀法提取阿胶糕中的蛋白质，胰蛋白酶进行酶解处理，利用超高效液相色谱-三重四级杆质谱法（UHPLC-QQQ MS），电喷雾离子源（ESI），正离子模式下扫描，采用多重反应监测模式，对特征肽目标离子进行检测。结果：经验证，鉴别方法和检查方法均均有良好的专属性，灵敏度高。市售样品测定结果显示，部分样品中检出阿胶成分，有的样品中检出牛皮源成分，有的样品中未检出任何皮源性成分。结论：所建立方法操作简便，专属性强，可用于阿胶糕中阿胶的鉴别和掺假杂皮胶的检测。 |
| English Summary: |
| To establish a method for the identification of colla corii asini in donkey-hide gelatin cake and the detection of constituents from horse，ox，sheep or pig skin with characteristic peptide as the detection marker.Methods:The protein was extracted by trichloroacetic acid precipitation method，and trypsin was used for enzymatic hydrolysis.UHPLC-QQQ MS was used with electrospray positive ionization mode and multiple reaction monitoring(MRM）to detect the target ions.Results:The method was validated to be specific and sensitive.The results showed that some samples were found to be containing colla corii asini and some samples containing bovine skin ingredients，whereas some samples did not detect any skin-derived constituents.Conclusion:The established method was validated to be simple and specific.It can be used for identification of colla corii asini in donkey-hide gelatin cake and detection of adulteration. |
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