世界中医药

作者	单位
吴玲，唐宇，郑琴，吴海霞，胡鹏翼，郭园园，杨婷，王梅，杨明	江西中医药大学现代中药制剂教育部重点实验室，南昌，330004

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中文摘要:

目的：研究川芎挥发油对抑郁模型大鼠行为学及脑内多巴胺、去甲肾上腺素的影响。方法：将72只SD雄性大鼠随机分为空白组、模型组、阳性药组、川芎挥发油低、中、高剂量组，通过应激实验建立大鼠CUMS抑郁模型，对其进行相关治疗后，考察行为学变化，同时采用LC-MS/MS检测脑组织中DA、NE含量。结果：与模型组比较，各剂量川芎挥发油组均能不同程度改善CUMS抑郁模型大鼠旷场实验水平得分和糖水偏爱百分比，减少强迫游泳静止不动时间和增加大鼠体质量；川芎挥发油中剂量组可极显著提高CUMS抑郁模型大鼠海马区DA的含量（P<0.01），川芎挥发油低、高剂量组可显著增加CUMS抑郁模型大鼠海马区DA的含量（P<0.05）；川芎挥发油低剂量组可极显著增加CUMS抑郁模型大鼠纹状体NE的含量（P<0.01），川芎挥发油中、高剂量可显著增加CUMS抑郁模型大鼠前额叶和纹状体NE的含量（P<0.05）。结论：川芎挥发油能改善CUMS抑郁模型大鼠旷场实验水平得分和糖水偏爱百分比，减少强迫游泳静止不动时间和增加大鼠体质量，显著提高CUMS抑郁模型大鼠海马区DA含量，前额叶和纹状体NE的含量，表明川芎挥发油抗抑郁作用可能与提高前额叶、纹状体NE含量及海马区DA含量有关。

English Summary:

To study the effects of volatile oil of Rhizoma Ligustici Chuanxiong on behavior,brain dopamine and norepinephrine in rats with depression.Methods：A total of 72 male Sprague-Dawley rats were randomly divided into a blank group,a model group,a positive drug group,and a low,medium and high dose group of volatile oil from Rhizoma Ligustici Chuanxiong.The CUMS depression model was established by stress test.After treatment,the behavioral changes were examined,and the contents of DA and NE in brain tissue were detected by LC-MS/MS.Results：Compared with the model group,each dose of volatile oil of Rhizoma Ligustici Chuanxiong could improve the level of sputum test and the percentage of sucrose preference in the CUMS depression model rats,reduce the resting time of forced swimming and increase the body weight of rats.The medium group could significantly increase the content of DA in hippocampus of CUMS depression model rats(P<0.01).The low and high doses of Rhizoma Ligustici Chuanxiong volatile oil could significantly increase the content of DA in hippocampus of CUMS depression model rats(P<0.05).The medium and high dose group could significantly increase the content of striatum NE in CUMS depression model rats(P<0.01).The high and medium doses of Rhizoma Ligustici Chuanxiong volatile oil can significantly increase the content of prefrontal and striatum NE in CUMS depression model rats(P<0.05).Conclusion：The volatile oil of Rhizoma Ligustici Chuanxiong can improve the open field test score and the percentage of sugar water preference in CUMS depression model rats,reduce the static immobility time and increase the body weight of rats,and significantly increase the DA content,prefrontal lobe and the content of striatum NE in hippocampus of CUMS depression model rats,which showed that the antidepressant effect of volatile oil of Rhizoma Ligustici Chuanxiong may be related to the increase of NE content in prefrontal cortex and striatum and DA content in hippocampus.

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