世界中医药

目的：探究对于超容量负荷作用下的大鼠心力衰竭模型使用中药强心方在急性期与慢性期的相关影响。方法：取45只SD大鼠随机均分为A组(假手术组)、B组(单纯造瘘心脏超负荷组)与C组(心脏超负荷+强心方观察组)，制备超容量负荷大鼠心力衰竭模型，并给予相应的处理与干预措施，急性期(3 d)进行氧化应激、基质金属蛋白酶(MMP)等生物化学指标检测，远期(8周)评价左心室的大小与功能。结果：B组大鼠的左心室肥大细胞密度显著高于A组与C组(P<0.05)；B组大鼠肥大细胞密度增加与基质金属蛋白酶2(Matrix Metalloprotein2，MMP2)活性增加呈显著相关性；容量超载使大鼠心肌氧化应激水平显著升高，B组平均水平显著高于A组与C组(P<0.05)，心室的重量显著大于A组(P<0.05)。与A组大鼠比较，右心室与肺组织重量亦显著增加，然而C组显著降低左右心室与肺组织的重量增加；与B组比较，C组大鼠整体的体质量降低(P<0.05)。与A组大鼠V0数据比较，B组大鼠存在显著左心室扩张(P<0.05)；强心方治疗能够显著降低左心室容积增加的程度(P<0.05)；心肌顺应性结果显示强心方可使其增加程度显著降低，与B组比较，差异无统计学意义(P<0.05)；收缩期的压力-容积数据提示随心脏超负荷的持续加载可见心室内收缩显著受抑制(P<0.05)，C组大鼠收缩功能与A组基本一致。结论：本研究中结果初步显示对于超容量负荷作用下诱导的大鼠心力衰竭动物模型，强心方具有明显的功能性心脏保护作用，并且这种保护作用可能与MMP活性与氧化应激反应的抑制相关。

English Summary:

To explore the effects of applyingQiangxin Formula of traditional Chinese medicine(TCM）in acute and chronic phases of rat heart failure models under overload.Methods：A total of 45 Sprague-Dawley(SD）rats were randomly divided into 3 groups：group A(sham operation),group B(simple ostomy cardiac overload）and group C(cardiac overload+Qiangxin Formula treatment).The model of heart failureinduced by overload was made and the corresponding treatment were applied.Biochemical indexes such as oxidative stress and matrix metalloproteinases were detected in the acute phase(3 days),and the left ventricular size and function were evaluated in the long term(8 weeks).Results：The density of left ventricular mast cells in the group B was significantly higher than that in the group A and C(P<0.05).The increase of mast cell density in the group B was significantly correlated with the increase of MMP-2 activity.The level of oxidative stress was increased significantlyunderoverload,in which the average level of the groupB was significantly higher than that of the group Aand C.The weight of the ventricle was significantly higher than that of the groupA; the weight of the right ventricle and the lung tissue was also significantly increased compared with the groupA,however,Qiangxin Formula could significantly reduce the weight gain of the left and right ventricles and lungs; Qiangxin Formula could reduce the overall body weight of the rat compared with the group B(P<0.05).Compared with the V0 data of the group A,there was significant left ventricular dilatation in the group B; Qiangxin Formula could significantly reduce the increase of left ventricular volume(P<0.05); myocardial compliance showed that Qiangxin Formula could reduceitsincrease(P<0.05 compared withthe group B).The systolic pressure-volume data suggested that the ventricular contraction was significantly inhibited as the cardiac overload was continuously loading(P<0.05),and thecontractive functionsof rats in the groupC andA were basically the same.Conclusion：The preliminary results of the study showed that Qiangxin Formula has a significant functional cardioprotective effect on rat models of heart failure induced by overload,and this protective effect may be related to the inhibition of MMP activity and oxidative stressreaction.

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