| 引用本文:肖红妮，刘志杰.“三棱-莪术”组分配伍介导PI3K-AKT通路治疗大鼠子宫肌瘤的研究[J].世界中医药,2019,(09):. |
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| “三棱-莪术”组分配伍介导PI3K-AKT通路治疗大鼠子宫肌瘤的研究 |
| Study on PI3K-AKT Pathway Mediated by “Rhizoma Sparganii-Rhizoma Sparganii” Components in the Treatment of Rat Uterine Leiomyoma |
| 投稿时间：2019-07-15 |
| DOI：10.3969/j.issn.1673-7202.2019.09.009 |
| 中文关键词: 三棱-莪术 组分配伍 PI3K-AKT通路 大鼠 子宫肌瘤 子宫系数 卵巢系数 子宫横竖径 |
| English Keywords:Rhizoma Sparganii-Rhizoma Sparganii Component compatibility PI3K-AKT pathway Rat Uterine leiomyoma Uterine coefficient Ovarian coefficient Transverse and vertical diameter of uterus |
| 基金项目:国家自然科学基金项目(81341100);甘肃省卫生行业科研计划项目(GSWSKY-2015-24) |
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| 中文摘要: |
| 目的:研究三棱-莪术对治疗子宫肌瘤的疗效及可能作用机制。方法:36只雌性SD大鼠随机分为空白组、模型组、1：1组、1：2组,每组9只,其中模型组、1：1组、1：2组制备子宫肌瘤模型,空白组、模型组用生理盐水灌胃,1：1组、1：2组根据三棱-莪术配伍比例进行灌胃,干预结束后比较各组子宫/卵巢系数、子宫大小,子宫病理形态以及PI3K/AKT通路标志因子的表达的变化。结果:1)模型组子宫平滑肌出现明显的病变,三棱-莪术干预后大鼠子宫组织病变有所改善,其中1：1组改善较1：2组明显。2)子宫肌瘤大鼠子宫系数、卵巢系数、子宫横竖径、子宫组织中E2和ER、PI3K、p-AKT/AKT表达水平均明显增加(P<0.05),经三棱-莪术干预后上述指标下降,其中术1：1组改善较1：2组明显(P<0.05)。结论:三棱-莪术配伍可改善子宫肌瘤,这一作用机制可能通过激活PI3K/AKT通路实现。 |
| English Summary: |
| To study the therapeutic effect and possible mechanism of Rhizoma Sparganii-Rhizoma Sparganii in the treatment of uterine leiomyoma.Methods:A total of 36 female SD rats were randomly divided into a blank group,a model group,a 1：1 group and a 1：2 group,with 9 rats in each group.The model group,1：1 group and 1：2 group were used to prepare uterine myoma model.The blank group and the model group were given normal saline by gastric lavage.The 1：1 group and 1：2 group were given gastric lavage according to the compatibility ratio of trigonum and zedoary turmeric.After intervention,the changes of uterus/ovary coefficient,uterine size,uterine pathological morphology and the expression of PI3K/AKT pathway marker factors were compared in each group.Results:1)The uterine smooth muscle in the model group showed obvious pathological changes,and the uterine tissue lesions improved after the intervention of Rhizoma Sparganii-Rhizoma Sparganii,and the improvement in the 1：1 group was more obvious than that in the 1：2 group.2)The uterine coefficient,ovarian coefficient,transverse and vertical diameter of uterus,and the expression levels of E2,ER,PI3K and p-AKT/AKT in uterine tissue of rats with uterine leiomyoma significantly increased(P<0.05).The above indexes decreased after intervention with Rhizoma Sparganii-Rhizoma Sparganii.The improvement in 1：1 group was more obvious than that in 1：2 group(P<0.05).Conclusion:The compatibility of Rhizoma Sparganii-Rhizoma Sparganii can improve uterine leiomyoma,and this mechanism may be achieved by activating PI3K/AKT pathway. |
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