| 引用本文:白俊其，黄娟，徐文，张靖，宫璐，黄志海，丘小惠.LC-MS/MS法测定石斛碱在大鼠体内药代动力学研究[J].世界中医药,2019,(09):. |
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| LC-MS/MS法测定石斛碱在大鼠体内药代动力学研究 |
| Pharmacokinetics of Dendrobin in Rats by LC-MS/MS |
| 投稿时间：2017-05-09 |
| DOI：10.3969/j.issn.1673-7202.2019.09.010 |
| 中文关键词: 石斛碱 药代动力学 血药浓度 LC-MS/MS |
| English Keywords:Dendrobine Pharmacokinetics Blood Concentration LC-MS/MS |
| 基金项目:广东省中医药管理局项目(2015KT1125)——基于药代动力学和代谢转化对石斛碱体内代谢的研究，负责人：白俊其 |
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| 中文摘要: |
| 目的：建立LC-MS/MS分析方法测定大鼠血浆中石斛碱浓度，考察其体内药代动力学特征。方法:SD大鼠6只灌胃给予石斛碱（20 mg/kg），于给药前及给药后5、15、30、45、60、90.120、180、240、420、600、1 440 min眼内眦静脉从取血，测定大鼠血浆石斛碱浓度。用DAS2.0统计软件计算药动学参数。结果:石斛碱浓度在0.5～50 ng/mL范围内线性关系良好（r2=0.999 2），定量下限为0.5 ng/mL，低（1 ng/mL）、中（5 ng/mL）、高（20 ng/mL）3个浓度的回收率分别为（78.86±4.56）%、（85.25±1.23）%及（80.56±2.23）%；日内及日间RSD均小于10%。石斛碱给药后血药浓度-时间曲线符合二室模型，消除半衰期为t1/2为(51.27±2.65)min，Tmax为(10.00±0.17)min，Cmax为(0.12±0.08)mg/L，AUC(0-∞)为（7.91±0.25）mg/L·min。结论：本分析方法对石斛碱体内外检测具有专属性，且灵敏、可靠，适用于石斛碱药代动力学研究。 |
| English Summary: |
| To establish an LC-MS/MS method for determination of dendrobine, and to study the pharmacokinetics of dendrobine in rats.Methods:Six male rats were orally administered with Dendrobium(20 mg/kg),Plasma samples were collected at 5、15、30、45、60、90.120、180、240、420、600、1440 min after the drug administration.The pharmacokinetic parameters were calculated by DAS2.0 software.Results:The calibration curves were linear over the concentration ranging from 0.5 to 50 ng/mL（r2=0.9992）with the LLOQ of 0.5 ng/mL.The intraday and interday RSD were generally good(<10%).The concentration-time curve for dendrobine was in accordance with the two-compartment model, The main pharmacokinetic parameters t1/2, Tmax, Cmax, AUC(0-∞)were as follows:(51.27±2.65)min,(10.00±0.17)min,(0.12±0.08)mg/L, （7.91±0.25）mg/L·min.Conclusion:The analytical method developed is sensitive specific rapid and reproducible and is proved to be suitable for the pharmacokinetic study of dendrobine. |
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