| 引用本文:白晶，高永红，高颖.培元通脑胶囊对CI大鼠工作记忆及海马内谷氨酸及其受体表达的影响[J].世界中医药,2019,(09):. |
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| 培元通脑胶囊对CI大鼠工作记忆及海马内谷氨酸及其受体表达的影响 |
| Effects of Peiyuan Tongnao Capsules on Working Memory,Glu in Hippocampus and Expression of Its Receptor in Rats with Cerebral Ischemia |
| 投稿时间：2018-09-10 |
| DOI：10.3969/j.issn.1673-7202.2019.09.012 |
| 中文关键词: 培元通脑胶囊 脑缺血 工作记忆 谷氨酸 N-甲基-D-天冬氨酸受体2B |
| English Keywords:Peiyuan Tongnao Capsules Cerebral ischemia Working memory Glu NMDA receptor 2B |
| 基金项目:北京中医药大学东直门医院企业横向科研项目(HX-DZM-2018009)——培元通脑胶囊动物实验研究 |
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| 中文摘要: |
| 目的:探讨培元通脑胶囊对脑缺血大鼠工作记忆行为及海马区谷氨酸(Glu)含量、N-甲基-D-天冬氨酸受体2B(NMDA Receptor 2B,NR2B)的影响。方法:将52只雄性SD大鼠按随机数字表法分为假手术组、模型组、尼莫地平组和培元通脑组,每组均13只。模型组、尼莫地平组和培元通脑组均将双侧颈内动脉结扎制备大鼠脑缺血模型。尼莫地平组和培元通脑组每日分别按体质量给予相应的药物灌胃共治疗4周。采用Morris水迷宫实验观察大鼠的工作记忆功能;尼氏染色法观察大鼠海马组织尼氏体表达情况;高效液相色谱法检测海马区Glu含量变化,Westernblot法检测大鼠海马区NR2B蛋白表达。结果:Morris水迷宫工作记忆逃避潜伏期假手术组大鼠（第1天与第2天比较、第1天与第3天比较P>0.05，第1天与第4天比较P<0.05）;模型组大鼠（第1天与第2天比较、第1天与第3天比较P>0.05，第1天与第4天比较P<0.05）;尼莫地平组大鼠（第1天与第2天比较、第1天与第3天比较P>0.05，第1天与第4天比较P<0.05）;培元通脑组大鼠（第1天与第2天比较、第1天与第3天比较P<0.05，第1天与第4天比较P<0.01）。海马内谷氨酸含量假手术组与模型组比较(P<0.05),模型组与培元通脑组比较P<0.05。NMDA2B受体蛋白表达比较假手术组与模型组、尼莫地平组、培元通脑组比较，差异有统计学意义(P<0.01),模型组与尼莫地平组比较(P<0.05)与培元通脑组比较(P<0.01)。结论:培元通脑胶囊能改善脑缺血大鼠的工作记忆能力,保护神经元细胞,其机制可能与上调海马区Glu、NR2B表达有关。 |
| English Summary: |
| To explore the effects of Peiyuan Tongnao(PYTN)Capsules on working memory,content of Glu in hippocampus and NMDA Receptor 2B(NR2B)in rats with cerebral ischemia(CI).Methods:A total of 52 male SD rats were randomly divided into sham operation group(n=13),model group(n=13),nimodipine group(n=13)and PTNY group(n=13).The latter 3 groups were performed with bilateral carotid artery ligation.The nimodipine group and PTNY group were given corresponding drugs daily by gavage for 4 weeks according to body weight.The working memory of the rats was was tested by Morris water maze.The expression of Nissl body in hippocampus was observed by Nissl staining.Determination of Glu in hippocampus was conducted by High Performance Liquid Chromatography.The expression level of NR2B was determined by Western blot.Results:Morris water maze working memory escape latency:the sham operation group(DAY 1 versus DAY 2,DAY 1 versus DAY 3,P>0.05; DAY 1 versus DAY 4,P<0.05); the model group(DAY 1 versus DAY 2,DAY 1 versus DAY 3,P>0.05,DAY 1 versus DAY 4,P<0.05); the nimodipine group(DAY 1 versus DAY 2,DAY 1 versus DAY 3,P>0.05,DAY 1 versus DAY 4,P<0.05); the PYTN group(DAY 1 versus DAY 2,P>0.05,DAY 1 versus DAY 3,DAY 1 versus DAY 4,P<0.05).The content of Glu in hippocampus:the sham operation group was significantly different from the model group(P<0.05); the model group was significantly different from the PYTN group(P<0.05).The expression of NR2B:the sham group was significantly different from the model group,the nimodipine group and the PYTN group(P<0.01); the model group was significantly different from the nimodipine group(P<0.05); the model group was significantly different from the PYTN group(P<0.01).Conclusion:PYTN Capsules were helpful for improving working memory and protecting neurons in rats with cerebral ischemia,whose mechanism may associate with increasing Glu in hippocampus and the expression of NR2B in hippocampus. |
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