| 引用本文:唐小铁，徐洁，王丽媛，于洋，崔学彬，孙维言.雷公藤甲素对糖尿病肾病小鼠肾足细胞自噬及调亡的影响[J].世界中医药,2019,(10):. |
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| 雷公藤甲素对糖尿病肾病小鼠肾足细胞自噬及调亡的影响 |
| Effect of Triptolide on Autophagy and Apoptosis of Renal Podocytes in Diabetic Nephropathy Mice |
| 投稿时间：2019-04-15 |
| DOI：10.3969/j.issn.1673-7202.2019.10.017 |
| 中文关键词: 糖尿病肾病 雷公藤甲素 足细胞 Podocin Bax Caspase-3 Lc3 |
| English Keywords:Diabetic nephropathy Triptolide Sertoli cell Podocin Bax Caspase-3 Lc3 |
| 基金项目:湖北省卫计委联合基金面上项目(WJ2018H0107);武汉市卫计委一般项目(WX17D27) |
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| 中文摘要: |
| 目的:探讨雷公藤甲素对糖尿病肾病(DN)小鼠肾足细胞自噬及调亡的影响。方法:选择小鼠60只,随机分为DN组和雷公藤甲素组各30只,光镜下观察各组肾组织病理学改变情况,观察肾功能变化情况,对其行行威廉姆斯肿瘤蛋白1(WT-1)免疫组化染色,观察足细胞情况,透射电镜观察足细胞自噬情况,采用Western blot法检测肾组织中Podocin、Lc3、Bax及Caspase-3的表达情况。结果：PAS染色结果显示,DN组小鼠系膜基质明显增多,雷公藤甲素组小鼠系膜基质明显减少。WT-1免疫组织化学染色显示,雷公藤甲素组肾足细胞数量比DN组明显增多。Western Blot结果显示,与DN组小鼠比较,雷公藤甲素组Podocin表达明显增加,LC3-II/LC3-I比值明显上升,凋亡相关蛋白Bax及Caspase-3表达明显减少(P<0.05)。结论:雷公藤甲素可减轻足细胞损伤,延缓DN的进展,可DN的治疗起到参考价值。 |
| English Summary: |
| To investigate the effects of triptolide on autophagy and apoptosis of renal podocytes in diabetic nephropathy(DN)mice.Methods:A total of 60 mice were randomly divided into a DN group and a triptolide group,with 30 mice in each group.The pathological changes of kidney tissues and renal function were observed under light microscope.The the expression of podocin,Lc3,Bax and Caspase-3 in kidney tissues were detected by Western blot and immunohistochemical staining of Williams Tumor Protein-1(WT-1)and podocyte autophagy were observed under transmission electron microscope.Results:PAS staining showed that the mesangial matrix increased significantly in DN group and decreased significantly in triptolide group.WT-1 immunohistochemical staining showed that the number of renal podocytes in triptolide group was significantly higher than that in DN group.Western Blot results showed that compared with DN mice,the expression of Podocin,LC3-II/LC3-I ratio,Bax and Caspase-3 in triptolide group increased significantly,while the expression of apoptosis-related proteins decreased significantly(P<0.05).Conclusion:Tripterygium wilfordii can alleviate podocyte injury and delay the progress of DN,which can be used as a reference for the treatment of DN. |
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