世界中医药

基金项目:财政部中央本级专项(2060302);国家重点研发计划项目(2017YFC1700701,2018YFF0214202);国家自然科学基金重大项目(81891014);国家自然科学基金青年基金项目(81603241);浙江省基础公益研究计划项目(LGN18H280003);宁波市科技创新团队项目(2015C110018)

作者	单位
杨健1，周利1，胡玲2，解会群3，余意4，刘汉伟2，何雅莉1，彭华胜3，郭兰萍1	1 中国中医科学院中药资源中心道地药材国家重点实验室培育基地,北京,100700 2 宁波检验检疫科学技术研究院,宁波,315000 3 安徽中医药大学药学院,合肥,230012 4 无限极(中国)有限公司,广州,510663

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中文摘要:

目的:建立采用电感耦合等离子体质谱法测定中药材何首乌中40种无机元素的方法,该方法的建立为何首乌药材的质量控制及优质资源的开发提供依据。方法:采用压力罐消解法对样品进行消解,利用电感耦合等离子体质谱法测定何首乌药材中40种无机元素的含量,并对检测方法进行方法学考察。结果:40种无机元素检测方法经方法学考察,结果合理有效,标准曲线的线性相关系数均>0.95,平均加样回收率在88.9%～119.2%,RSD均<12%.测定不同来源的49批何首乌样品,K,Ca,Mg,Al,Fe等5种元素含量最高,均达到100 mg/kg以上;Be,Sc,Ge,Eu,Tb,Ho,Tm,Yb和Lu等8种无机元素的平均含量较低,均<0.02 mg/kg;不同产地的何首乌样品表现出一定的聚类性特征。结论:该方法操作简便,快速、准确,结果可靠,适用于中药材何首乌中无机元素的测定。

English Summary:

To establish a method for the simultaneous determination and analysis of 40 inorganic elements in Polygoni Multiflori Radix.by using Inductively Coupled Plasma Mass Spectrometry(ICP-MS)，and provide an experimental basis for improving the quality standard and developing excellent resources of Polygoni Multiflori Radix.Methods:40 inorganic elements in Polygoni Multiflori Radix were determined by using high pressure digestion and ICP-MS.And the methods were validated.Results:The methods of 40 inorganic elements were reasonable and effective by validation.The standard curves were linear and the linear correlation coefficients were greater than 0.95.The recovery was between 88.9%～119.2%，and RSD were all lower than 12%.In the determination of 49 batches of Polygoni Multiflori Radix samples from different regions，5 elements of K，Ca，Mg，Al，Fe were higher than 100 mg/kg，and 8 elements of Be，Sc，Ge，Eu，Tb，Ho，Tm，Yb and Lu were lower than 0.2 mg/kg; and the samples from different regions showed some clustering characteristics.Conclusion:The established method is simple with fast analysis and high sensitivity，and the method is suitable for determination of inorganic elements in Polygoni Multiflori Radix.

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