| 引用本文:史琦，李春雷，孔艳华，龙泓竹，李阳溪，阎玥，李友林.SD大鼠哮喘模型建立方法及评价的比较研究[J].世界中医药,2019,(11):. |
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| SD大鼠哮喘模型建立方法及评价的比较研究 |
| Comparative Study on the Establishment Method and Evaluation of SD Rat Asthma Model |
| 投稿时间：2018-11-06 |
| DOI：10.3969/j.issn.1673-7202.2019.11.014 |
| 中文关键词: SD大鼠 哮喘 造模 模型评价 |
| English Keywords:SD rats Asthma Model building Model evaluation |
| 基金项目:国家自然科学基金青年科学基金项目(81403377);北京市科学技术委员会“十病十药”研发项目(Z161100001816004) |
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| 中文摘要: |
| 目的:观察不同致敏液成分、致敏液注射部位、激发液浓度、激发时长所造成的SD大鼠哮喘模型的差异,旨在建立一种较为成功的哮喘大鼠模型。方法:分别以10% OVA/Al(OH)3混合液1 mL和10%OVA/Al(OH)3+百日咳杆菌混合液1 mL作为致敏液;注射部位分为腹腔注射和五点注射(双侧足心、双侧腹股沟皮下、腹腔);以1%OVA或2%OVA作为激发液;激发时间包括7 d和21 d;经上述方法不同组合叠加造模后评价大鼠的一般活动状态、肺组织病理及肺功能检测结果。结果:OVA+百日咳腹腔注射组和OVA+百日咳五点注射组大鼠出现呼吸急促,呼吸困难等典型的哮喘症状,OVA腹腔注射组1部分大鼠出现较轻程度的哮喘症状,其余各组哮喘症状不明显。病理结果显示:OVA+百日咳五点注射组大鼠可见大量的嗜酸性粒细胞和淋巴细胞浸润、管腔狭窄、黏液栓生成、支气管痉挛;OVA腹腔注射组1和OVA+百日咳腹腔注射组主要表现为淋巴细胞浸润和杯状细胞较多;其余各组病理改变不明显。肺功能结果显示:叠加了百日咳后的腹腔注射组和五点注射组大鼠的I值和Rn值均较单纯OVA腹腔注射组升高,五点注射组大鼠的气道高反应性升高更加明显(P<0.01)。结论:OVA结合灭活百日咳杆菌共同致敏、多点皮下注射联合腹腔注射能比较成功的构建SD大鼠哮喘模型。 |
| English Summary: |
| To observe the differences of SD rat asthma models with different methods of sensitization fluid composition, sensitized injection site, atomization liquid concentration and time, aiming to establish a more successful asthma rat model. Methods:The10% OVA/Al(OH)3 mixture 1 mL or 10% OVA/Al(OH)3+ pertussis mixture 1 mL were taken as the sensitization fluid respectively; sensitized injection site was abdominal injection or 5 points injection (bilateral foot heart, bilateral inguinal subcutaneous and abdominal injection); using 1% OVA or 2% OVA as the excitation liquid; excitation time including 7 days and 21 days. General activities, pulmonary pathology and pulmonary function detection were evaluated by different combination methods of factors above. Results:Typical asthmatic symptoms such as shortness of breath, dyspnea and so on in OVA+ pertussis abdominal injection group and OVA+ pertussis 5 points injection group rats were obvious. Mild asthma symptoms were observed in the first OVA group rats, and asthma symptoms in other groups were not obvious. The pathological results showed that OVA+ pertussis 5 points injection group rats showed a large number of eosinophils and lymphocytes infiltration, bronchostenosis, mucus production and bronchospasm. The first OVA group and the OVA+ pertussis abdominal injection group rats showed infiltration of lymphocytes and goblet cells. The pathological changes in the other groups were not obvious. Results of lung function showed that: I and Rn values of OVA+ pertussis 5 points injection group and OVA+ pertussis abdominal injection group rats increased compared with those of the OVA abdominal injection group. Hyperresponsiveness of OVA+ pertussis 5 points injection group increased more significantly (P<0.01). Conclusion:Sensitization with both OVA and pertussis, multipoint subcutaneous injections combined with abdominal injection can successfully construct the asthma model in SD rats. |
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