世界中医药

目的:探讨右归丸对肾阳虚模型大鼠下丘脑-垂体-肾上腺轴的影响。方法:将60只健康雄性SD大鼠随机分为右归丸组、模型组和空白组,每组20只。右归丸组大鼠接受肾阳虚模型制备后予右归丸灌胃给药干预;模型组大鼠接受肾阳虚模型制备后接受右归丸组同体积的0.9%NaCl灌胃,连续灌胃20 d;空白组大鼠不接受造模制备,第16日起同右归丸组同体积的0.9%Nacl灌胃,连续灌胃20日。造模结束后每隔4日常规记录大鼠体质量、肛温、饮水量、尿量、以及抓取激惹反应。比较3组大鼠促肾上腺皮质激素释放因子(CRF)、促肾上腺皮质激素(ACTH)、大鼠皮质醇(CORT)、促肾上腺皮质激素释放激素受体1(CRHR1)的含量变化。结果:1)造模后发现接受模型制备的大鼠表现为肛温降低,饮水量及摄食量减少,体质量下降,大便溏薄,尿量增多,抓取激惹反应减弱。进行药物干预后,右归丸组大鼠的肛温、饮水量、摄食量、抓取激惹反应、体质量均高于模型组比较，差异有统计学意义(P<0.05),尿量减少,且大便干湿趋于正常;2)与空白组比较模型组及右归丸组肾上腺、下丘脑、垂体的脏器指数均下降(P<0.05),其中模型组下降的趋势更为明显,右归丸组较模型组肾上腺、下丘脑、垂体的脏器指数有所上升(P<0.05);3)与空白组比较,模型组及右归丸组大鼠接受造模后血清CRF、ACTH、CORT以及肾上腺、下丘脑、垂体的CRHR1含量均明显下降,经过右归丸干预后,该组别的大鼠CRF、ACTH、CORT、CRHR1含量均得到提升,其中CRF、ACTH含量接近空白组水平。4)与空白组比较,模型组及右归丸组大鼠接受造模后肾上腺、下丘脑、垂体的CRF、ACTH、CORT及CRHR1 mRNA水平均明显下降,经过右归丸干预后,该组别的大鼠CRF、ACTH、CORT、CRHR1mRNA水平均得到提升。结论:右归丸可能是通过调节下丘脑-垂体-肾上腺皮质轴的功能发挥温肾补阳的作用。

English Summary:

To explore the effects of Yougui Pills on hypothalamus-pituitary-adrenal cortex axis in the kidney-yang deficiency model rats.Methods:A total of 60 healthy male SD rats were randomly divided into a Yougui Pills group，a model group and a blank group，with 20 cases in each group.After the kidney-yang deficiency model establishment，the rats of Yougui Pills group had Yougui Pills by intragastric administration; After the kidney-yang deficiency model establishment，the rats of the model group had the same volume 0.9% Nacl as Yougui Pills by intragastric administration，continuous for 20 days; The rats in the blank group did not accept model preparation，and had the same volume 0.9% Nacl as Yougui Pills by intragastric administration began with the 16th day，continuous for 20 days; After model establishment，the weight，anus temperature，water intake，urine volume，and grab irritability reaction of rats were recorded every 4 days.The content changes of corticotrophin releasing factor(CRF)，adrenocorticotropic hormone(ACTH)and cortisol(CORT)of rats，adrenocorticotropic hormone releasing hormone receptor 1(CRHR1)among the 3 groups of rats were compared.Results:1)after the model establishment，it was found that the established model rats had anal temperature reduction，drinking water and food intake decrease，weight loss，thin sloppy stool，urine volume increased，fetching irritability reaction weaken.After the pharmacological intervention，the rats of Yougui Pills group were higher than that in the model group in anal temperature，water intake，food intake，grab irritability reaction and weight.The comparison was with statistical differences(P<0.05)and the urine volume reduced and stool moisture returned to normal.2)Compared with the blank group，the indexes of adrenal gland，hypothalamus and pituitary decreased in the model group and the Yougui Pills group(P<0.05).The model group had more obvious decreased trend.The indexes of adrenal gland，hypothalamus and pituitary increased in the Yougui Pills group compared with model group(P<0.05).3)Compared with the blank group，the rats had model establishment in the model group and Yougui Pills group had the significantly reduction of serum CRF，ACTH，CORT and CRHR1 content of adrenal gland，hypothalamus and pituitary，after the intervention of Yougui Pills.CRF，ACTH，CORT，CRHR1 content of the rats in the group had improved.CRF and ACTH levels were close to the blank group levels.4)Compared with the blank group，the model established rats in the model group and Yougui Pills group had the significantly mRNA levels reduction of CRF，ACTH，CORT and CRHR1 in adrenal gland，hypothalamus and pituitary.After the Yougui Pills intervention，CRF，ACTH，CORT and CRHR1 mRNA levels were improved in the group.Conclusion:The Yougui Pills may exert the function of warming the kidney and tonifying yang by adjusting the hypothalamus-pituitary-adrenal cortex axis.

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