世界中医药
文章摘要
引用本文:胡文良,孙学威,郑艳秋.刺五加调控p38MAPK信号通路对顺铂所致小鼠耳毒性的防护作用[J].世界中医药,2019,(11):.  
刺五加调控p38MAPK信号通路对顺铂所致小鼠耳毒性的防护作用
Protective Effects of Radix et Caulis Acanthopanacis Senticosi on Cisplatin-Induced Ototoxicity in Mice by Regulating p38MAPK Signaling Pathway
投稿时间:2019-09-05  
DOI:10.3969/j.issn.1673-7202.2019.11.017
中文关键词:  刺五加  耳毒性  顺铂  p38MAPK信号通路  细胞凋亡
English Keywords:Radix et Caulis Acanthopanacis Senticosi  Ototoxicity  Cisplatin  p38MAPK signaling pathway  Apoptosis
基金项目:内蒙古医科大学科技百万工程项目(KJbw2013014)
作者单位
胡文良,孙学威,郑艳秋 内蒙古医科大学附属医院耳鼻喉头颈外科呼和浩特010000 
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中文摘要:
      目的:探讨刺五加对顺铂所致小鼠耳毒性的防护作用及对p38丝裂原活化蛋白激酶(p38 MAPK)信号通路的调控作用。方法:将BALB/c小鼠根据随机数字表法随机分为空白组、模型组和实验组,每组15只。除空白组小鼠外,其余3组小鼠均腹腔注射顺铂4 mg/kg,同时实验组预先给予腹腔注射刺五加4 mL/kg,空白组给予等量生理盐水干预,3组小鼠干预5 d后进行指标的检测及评价。检测3组小鼠听性脑干反应(ABR)阈值及耳蜗组织细胞凋亡情况,蛋白免疫印迹法检测3组小鼠耳蜗组织细胞p38MAPK信号通路相关蛋白表达情况。结果:与空白组比较,模型组8 Hz、12 Hz、24 Hz小鼠ABR阈移升高,差异有统计学意义(P<0.05),与模型组比较,实验组ABR阈移降低,差异有统计学意义(P<0.05)。空白组、模型组和实验组小鼠螺旋神经节细胞凋亡指数(AI)分别为(2.33±0.54),(19.76±0.84),(6.97±0.77),蛋白免疫印迹法检测显示,空白组、模型组及实验组螺旋神经节p-p38MAPK蛋白相对表达分别为(0.22±0.03)、(0.93±0.02)、(0.64±0.04);磷酸化环磷腺苷反应元件结合蛋白(p-CREB)相对表达分别为(0.19±0.04)、(0.84±0.05)、(0.43±0.02),与模型组比较,空白组和实验组AI及螺旋神经节p-p38MAPK、p-CREB蛋白表达差异有统计学意义(P<0.05)。结论:刺五加可对顺铂所致小鼠耳毒性起到保护作用,其机制与调控p38MAPK信号通路蛋白表达抑制耳蜗细胞凋亡程序的发生有关。
English Summary:
      To investigate the protective effect of Radix et Caulis Acanthopanacis Senticosi on cisplatin-induced ototoxicity in mice and the regulation of p38MAPK signaling pathway.Methods:According to body mass stratification,BALB/c mice were divided into a blank group,a model group and an experimental group,with 15 mice in each group.In addition to the blank group,all the mice in the other groups were intraperitoneally injected with DDP 4 mg/kg,while the experimental group was given the same amount of saline in advance by intraabdominal injection of Radix et Caulis Acanthopanacis Senticosi 4 mL/kg.The indexes were detected and evaluated after 5 days of intervention in each group.The threshold of auditory brainstem response(ABR)and apoptosis of cochlear tissue cells were detected.The expression of p38MAPK signal pathway-related protein in cochlear tissue cells was detected by Western blot.Results:Compared with the blank group,the ABR threshold shift in the model group increased at 8 Hz,12 Hz and 24 Hz(P<0.05),and compared with model group,ABR threshold in the experimental group decreased(P<0.05).The AI of spiral ganglion cells in the blank group,the model group and the experimental group were(2.33±0.54),(19.76±0.84),(6.97±0.77),respectively.WB detection showed that the relative expression of p-p38MAPK protein in spiral ganglion cells in the blank group,the model group and the experimental group were(0.22±0.03),(0.93±0.02),(0.64±0.04),and the relative expression of p-CREB protein was(0.19±0.04),(0.84±0.05),(0.43±0.02),respectively.Compared with the model group,the expression levels of AI and p-p38MAPK,p-CREB in the spiral ganglion of the blank group and the experimental group were significantly different(P<0.05).Conclusion:Radix et Caulis Acanthopanacis Senticosi has protective effect on cisplatin-induced ototoxicity in mice.Its mechanism is related to regulating the expression of p38MAPK signaling pathway and inhibiting the apoptotic process of cochlear cells.
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