| 引用本文:徐嘉一1,2，高彦彬1,2，邹大威1,2，王晓磊1,2，王涛1,2，师一民1,2，吴冰杰1,2.糖肾宁对高糖诱导的足细胞损伤模型Rho/ROCK通路的影响[J].世界中医药,2019,(12):. |
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| 糖肾宁对高糖诱导的足细胞损伤模型Rho/ROCK通路的影响 |
| Effects of Tangshenning on High Glucose-Induced Podocyte Injury Based on Rho/ROCK Pathway |
| 投稿时间：2019-06-28 |
| DOI：10.3969/j.issn.1673-7202.2019.12.014 |
| 中文关键词: 中药 糖肾宁 高糖 足细胞损伤 Rho/ROCK通路 实验研究 足细胞标志蛋白 糖尿病肾病 |
| English Keywords:Traditional Chinese medicine Tangshenning High glucose Podocyte Rho/ROCK signal pathway Experimental study Podocyte marker protein Diabetic Nephropathy |
| 基金项目:北京市自然科学基金项目（7172032）；国家自然科学青年基金项目（81302951）；科技部国家重点研发计划（2018YFC1704100） |
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| 中文摘要: |
| 目的：基于Rho/ROCK信号通路研究中药糖肾宁对高糖诱导足细胞损伤模型的影响。方法：高糖培养足细胞，以不同药物分组干预足细胞24 h，用罗丹明染色的鬼笔环肽法检测足细胞骨架，细胞免疫荧光检测足细胞标志蛋白Nephrin，Western Blotting检测RhoA、ROCK1、Nephrin蛋白的表达。比较不同药物或药物浓度对高糖诱导足细胞损伤模型的影响。结果：糖肾宁可改善足细胞骨架结构，下调高糖诱导的足细胞损伤模型中异常升高的RhoA和ROCK1蛋白表达，上调Nephrin表达。结论：中药糖肾宁可抑制高糖诱导的足细胞损伤模型RhoA/ROCK1信号通路，减轻足细胞骨架重构，改善足细胞损伤。 |
| English Summary: |
| To investigate the mechanism of traditional Chinese medicine Tangshenning on high glucose-induced podocyte injury based on Rho/ROCK signal pathway.Methods:Podocytes were cultured in high glucose.The podocytes were treated with different drugs for 24 h.The podocyte cytoskeleton was detected by rhodamine-stained phalloidin.The podocyte marker protein Nephrin was detected by immunofluorescence.Western blotting was used to detect RhoA, ROCK1 and Nephrin protein.Results:Tangshenning could improve the podocyte cytoskeleton, decreased expression of RhoA and ROCK1 protein, and increased expression of high glucose-induced Nephrin expression in high glucose-induced podocyte injury model.Conclusion:Traditional Chinese medicine Tangshenning can reduce the expression of RhoA/ROCK1 signaling pathway-related protein in high glucose cultured podocytes, reduce podocyte cytoskeletal remodeling, increase the expression of high glucose-induced Nephrin protein, and improve high glucose-induced podocyte injury. |
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