| 引用本文:郑琦，侯炜，王家伟，王雪凯，樊慧婷，林洪生.六神丸治疗大鼠放射性肺炎的机制研究[J].世界中医药,2019,(12):. |
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| 六神丸治疗大鼠放射性肺炎的机制研究 |
| Mechanism of Liushen Pills in Treating Rats with Radiation Pneumonitis |
| 投稿时间：2018-09-27 |
| DOI：10.3969/j.issn.1673-7202.2019.12.016 |
| 中文关键词: 六神丸 放射性肺炎 TNF-α IL-1β |
| English Keywords:Liushen Pills Radiation pneumonitis TNF-α IL-1β |
| 基金项目:国家自然科学基金项目(81603567）——基于STAT3/S100A8/A9信号通路探讨西黄丸通过调控骨髓来源免疫抑制细胞抑制肿瘤转移的研究 |
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| 中文摘要: |
| 目的:观察六神丸对放射性肺炎大鼠的治疗作用,对其血清中IL-1β、IL-6、TNF-α和TGF-β1进行检测,探究六神丸对放射性肺炎的治疗作用及可能的分子机制。方法:将40只雄性SD大鼠随机分为正常组、模型组、激素组、六神丸组,采用直线加速器对除正常组外的3组大鼠进行全胸单次照射,剂量为16 Gy。照射24 h后各组进行给药。照射后第4周,处死动物。取全肺称重计算肺系数;进行HE染色。采用Luminex法检测大鼠血清中细胞因子的表达。结果:与正常组比较,照射后大鼠肺系数显著升高(P<0.05),使用激素或六神丸干预后,大鼠肺系数较模型组低,差异有统计学意义(P<0.05,P<0.01)。HE结果显示激素组、六神组大鼠肺组织出现充血、水肿、渗出等炎性病理学改变均较模型组有改善,炎细胞浸润也不同程度减轻。六神丸可显著下调大鼠血清中IL-1β、TNF-α炎性反应因子的表达(P<0.05,P<0.01)。结论:放射性肺炎大鼠血清中炎性因子明显增加的增加,六神丸可有效减轻放射性肺炎大鼠局部肺损伤的程度,其机制可能与下调血清中TNF-α及IL-1β相关。 |
| English Summary: |
| To observe the effects of Liushen Pills on IL-1β, IL-6, TNF-α and TGF-β1 in the serum of rats with radiation pneumonitis(RP), and explore the therapeutic effect of Liushen Pills(LS)on RP and the possible molecular mechanism.Methods:A total of 40 male SD rats were divided into a normal control(NC)group, a model group, a prednisone acetate(PA)group, a LS group, randomly.Linear accelerator irradiated in the 3 groups except the NC group for single chest with the dose of 16Gy in rats.The rats in each group were given the drug by perfusion 24 hours after irradiation.After 4 weeks, the rats were sacrificed.The lung coefficient was calculated by weighing the lungs.The pathological changes in the lungs were observed by hematoxylin-eosin staining.The expressions of cytokines in serum of rats were detected by Luminex.Results:Compared with the NC group rats, the lung coefficient of model rats were significantly increased(P<0.05), while the lung coefficient of PA-treated and LS-treated rats were markedly decreased compared with the model rats, and the difference was statistically significant(P<0.05, P<0.01).The HE results showed that the PA-treated, LS-treated rats inflammatory lesions such as congestion, edema, and exudation were lighten than model rats in lung tissues.Liushen Pills could significantly decreased the expressions of IL-1β, TNF-α in the serum of rats(P<0.05, P<0.01).Conclusion:The proinflammatory cytokines in the serum of rats with RP were increased.Liushen Pills can effectively reduce lung injury in rats with RP, which may be related to the down-regulation of TNF-α and IL-1β in serum. |
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