世界中医药

目的:研究牛黄的主要成分牛磺酸(Taurine)对1型糖尿病小鼠主动脉僵硬度和内皮功能障碍的影响。方法:健康的雄性C57BL/6J小鼠30只,随机分为CON组、MD组和MD+牛磺酸组,每组10只。采用SphygmoCor脉搏分析系统分析主动脉脉搏流速(APV)m/s、主动脉脉搏波增强指数(API)和主动脉脉搏波增强指数(AIX)等指标反映主动脉僵硬度;采用氧化酶法、放射免疫法分别检测血清葡萄糖水平和胰岛素含量;根据血管周围组织内皮依赖性扩张(EDD%)和内皮独立扩张(EID%)评价主动脉内皮功能;采用RT-PCR法、Western blot方法对胸主动脉组织细胞eNOS mRNA表达水平及eNOS在不同处理组细胞中蛋白表达情况进行测定。结果:MD组(MD组)与正常对照组(CON组)比较,其主动脉内膜破坏严重,内皮细胞的体积增大,甚至部分脱落和间隙增大,中膜平滑肌细胞排列紊乱。与MD组比较,MD+TUDCA组(MD+牛磺酸)组主动脉壁各层细胞的排列较整齐,病变减轻。MD+牛磺酸组小鼠体质量及空腹血清胰岛素水平差异无统计学意义(P>0.05),而空腹血糖升高至(22.4±4.6）mmol/L,ISI降低(P<0.05);且APV、API和AIX均减轻或降低,差异有统计学意义(P<0.05)。与MD比较,MD+牛磺酸组eNOS mRNA表达上调,牛磺酸可提高eNOS蛋白水平的表达(P<0.01)。结论:长期服用牛磺酸对1型糖尿病小鼠主动脉僵硬度有改善作用,还可以通过增加机体一氧化氮活性和提高机体抗氧化能力从而预防小鼠内皮功能障碍,其可能是治疗及预防糖尿病血管功能障碍的有效药物。

English Summary:

To study the effects of the main component of Calculus Bovis, Taurine on aortic stiffness and endothelial dysfunction in type 1 diabetic mice.Methods:A total of 30 healthy male C57BL/6J mice were randomly divided into a CON group(n=10), a MD group(n=10)and a MD+ Taurine group(n=10).Aortic pulse velocity(APV)m/s, aortic pulse wave enhancement index(API)and aortic pulse wave enhancement index(AIX)were analyzed by SphygmoCor pulse analysis system to reflect aortic stiffness.Oxidase and radioimmunoassay were used to measure serum glucose levels and insulin levels, respectively.According to the endothelial-dependent dilation(EDD%)and endothelial-independent dilation(EID%), the endothelial functionob in peripheral tissues was evaluated.The expression level of eNOS mRNA in aortic tissue and expression of eNOS protein in cells of different treatment groups were measured by RT-PCR and Western blot.Results:Compared with the normal control group(CON group), the model group(MD group)mice showed more serious aortic intima destruction, the volume of endothelial cells was significantly increased, even partial shedding, and the arrangement of mesangial smooth muscle cells was disordered.Compared with the MD group, cells in the aortic wall of the sulfonated deoxycholic acid intervention group(MD+Taurine)were arranged orderly and the lesions were significantly reduced.There was no significant difference in body weight and fasting serum insulin level(P>0.05).However, fasting blood glucose was significantly increased to （22.4±4.6）mmol/L, while ISI, APV, API and AIX were reduced or decreased, with statistically significant differences(P<0.05).Compared with MD group, eNOS mRNA expression in MD+Taurine group was significantly increased.The results indicated that the expression level of eNOS protein was significantly increased by sulphuric deoxycholic acid and the difference was statistically significant(P<0.01).Conclusion:Sulfonic deoxycholic acid has an effect on aortic stiffness in type 1 diabetic mice, and it can prevent endothelial dysfunction in mice by increasing nitric oxide activities and increasing antioxidant capacity.It may be an effective medicine for the prevention and treatment of diabetic vascular dysfunction.

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