| 引用本文:陈亮，董长城.二氢丹参酮对人非霍奇金淋巴瘤Raji细胞增殖、凋亡的影响及机制[J].世界中医药,2019,(12):. |
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| 二氢丹参酮对人非霍奇金淋巴瘤Raji细胞增殖、凋亡的影响及机制 |
| Effects of Dihydrotanshinone on Proliferation and Apoptosis of Human Non-Hodgkin's Lymphoma Raji Cells and its Mechanism |
| 投稿时间：2019-09-27 |
| DOI：10.3969/j.issn.1673-7202.2019.12.023 |
| 中文关键词: 非霍奇金淋巴瘤 二氢丹参酮 增殖 凋亡 机制 |
| English Keywords:Human non-Hodgkin's lymphoma Dihydrotanshinone Proliferation Apoptosis Mechanism |
| 基金项目:内蒙古自治区自然科学基金项目（2017MS0815） |
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| 中文摘要: |
| 目的：探究二氢丹参酮对人非霍奇金淋巴瘤Raji细胞增殖、凋亡的影响及机制。方法：体外培养人非霍奇金淋巴瘤Raji细胞，并分为空白组和低/高剂量组，低/高剂量组以8、15 μmol/L二氢丹参酮处理，空白组以等量0.9%氧化钠注射液处理。用CCK-8法检测Raji细胞成活率，Annexin V-FITC/PI双染流式细胞术检测各组Raji细胞凋亡情况，试剂盒检测细胞中Caspase 8活性，免疫荧光法检测Raji细胞中CytC移位情况，蛋白免疫印迹法检测Raji细胞中Bad、Bax蛋白表达。结果：低/高剂量组药物干预24、48 h后细胞成活率及增殖指数低于空白组，差异有统计学意义（P<0.05）。干预48 h后，低/高剂量组Raji细胞凋亡率、Caspase 8活性及CytC表达量高于空白组，差异有统计学意义（P<0.05）。与空白组比较，低/高剂量组Bad蛋白表达升高，差异有统计学意义（P<0.05），Bax蛋白表达量差异无统计学意义（P>0.05）。结论：二氢丹参酮可有效抑制人非霍奇金淋巴瘤Raji细胞增殖，并诱导其发生凋亡，其作用机制与上调Caspase8的表达，诱导Bad表达有关，且呈剂量依赖性。 |
| English Summary: |
| To investigate the effects of dihydrotanshinone on proliferation and apoptosis of human non-Hodgkin lymphoma Raji cells and its mechanism.Methods:Raji cells of human non-Hodgkin's lymphoma were cultured in vitro and divided into a blank group and a low/high dose experimental group.The experimental group was treated with 8, 15 μmol/L dihydrotanshinone, and the blank group was treated with 0.9% NaCL.CCK-8 method was used to detect the survival rate of Raji cells, and Annexin V-FITC/PI double-staining flow cytometry was used to detect the apoptosis of Raji cells in each group.Caspase 8 activity was detected by kit, and Cytc translocation in Raji cells was detected by immunofluorescence, and Bad and Bax protein expression in Raji cells was detected by Western blotting.Results:The cell viability and proliferation index of low/high dose experimental group were lower than those of the blank group after 24 and 48 hours of drug intervention(P<0.05).After 48 hours of intervention, Raji cell apoptosis rate, Caspase 8 activity and Cytc expression in the low/high dose experimental group were higher than those in the blank group(P<0.05).WB detection showed that compared with the blank group, the expression of Bad protein in the low/high dose experimental group increased(P<0.05), and the expression of Bax protein had no significant difference(P>0.05).Conclusion:Dihydrotanshinone can effectively inhibit the proliferation and induce apoptosis of Raji cells in non-Hodgkin's lymphoma, and its mechanism is related to up-regulation of Caspase 8 expression and Bad expression, and with dose-dependence. |
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