| 引用本文:崔兰凤1，徐红日1，王玉婷2，赵世同2，王成祥1.扶正解毒化瘀方对脂多糖诱导大鼠肺巨噬细胞的调控作用[J].世界中医药,2020,(04):. |
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| 扶正解毒化瘀方对脂多糖诱导大鼠肺巨噬细胞的调控作用 |
| Study on Regulatory Effect of Fuzheng Jiedu Huayu Decoction on Lung Macrophages Induced by LPS in Rats |
| 投稿时间：2019-07-26 |
| DOI：10.3969/j.issn.1673-7202.2020.04.012 |
| 中文关键词: 扶正解毒化瘀方 多重耐药铜绿假单胞菌 NLRP3炎性体 固有免疫 脂多糖 |
| English Keywords:Fuzheng Jiedu Huayu Decoction Multidrug-resistant pseudomonas aeruginosa(MDRPA) NLRP3 inflammasome Innate immunity LPS |
| 基金项目:国家自然科学基金项目（81573924） |
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| 中文摘要: |
| 目的：观察扶正解毒化瘀方对脂多糖诱导的大鼠肺巨噬细胞中NLRP3炎性体通路及相关炎性反应递质的作用。方法：将大鼠NR8383细胞分为空白组、模型组、扶正解毒化瘀方组、哌拉西林他唑巴坦（TZP）组以及中药+TZP组。用制备扶正解毒化瘀方中药原液作用于脂多糖诱导的大鼠NR8383细胞，采用qPCR和Western blotting分别检测NLRP3炎性体通路的mRNA表达和蛋白水平，酶联免疫吸附试验（ELISA）检测干预前后炎性反应递质的水平。结果：扶正解毒化瘀方组和TZP组均能明显降低NLRP3、Caspase-1和ASC的mRNA表达，2组NLRP3和ASC的蛋白水平明显下降，Caspase-1和IL-1β蛋白水平也有下降趋势。扶正解毒化瘀方组和TZP组均能明显降低IL-18和IL-1β炎性反应递质水平，联合用药有增效作用。结论：扶正解毒化瘀方对NLRP3炎性体作用明确，可能通过NLRP3炎性体通路延缓机体免疫炎性损伤。 |
| English Summary: |
| To observe the effect of Fuzheng Jiedu Huayu Decoction on the NLRP3 inflammasome pathway and related inflammatory factors in rats' lung macrophages induced by LPS.Methods:Original liquid of Fuzheng Jiedu Huayu Decoction was prepared and applied on LPS-induced NR8383 rat cells.They were divided into blank group，model group，Fuzheng Jiedu Huayu Decoction group，piperacillin-tazobactam(TZP)group and combination group.The qPCR and western-blot were used to detect the expression of mRNA and protein level of the NLRP3 inflammasome pathway，respectively.ELISA was used to detect levels of inflammatory factors before and after the intervention.Results:Both Fuzheng Jiedu Huayu Decoction and TZP could significantly reduce the mRNA expressions of NLRP3，Caspase-1 and ASC.The protein levels of NLRP3 and ASC in the 2 groups were decreased significantly.The protein levels of Caspase-1 and IL-1β also showed a decreasing trend.Both Fuzheng Jiedu Huayu Decoction and TZP could significantly reduce the levels of IL-18 and IL-1β inflammatory factors.And the combination of drugs produced a synergistic effect.Conclusion:Fuzheng Jiedu Huayu Decoction has a certain effect on NLRP3 inflammasome，which may delay immune inflammatory injury of the body through the NLRP3 inflammasome pathway. |
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