世界中医药

目的：观察苦参碱对急性髓系白血病SCID小鼠模型细胞生物学特性的影响。方法：将40只数字随机法分为空白组（n=10）、模型组（n=30），其中模型组小鼠腹腔注射对数生长期HL-60细胞株制备急性髓系白血病模型，4周后将27只成模的模型组小鼠随机分为造模组、苦参碱A组及苦参碱B组，每组9只。其中苦参碱A组及B组分别用10 mL/kg及5 mL/kg灌胃，空白组及造模组大鼠接受等体积生理盐水灌胃，各组均连续干预4周。比较各组生存期、体质量、外周血血常规变化、同源性磷酸酶-张力蛋白（PTEN）水平变化、白血病细胞HL-60各周期生长情况以及Th1/Th2细胞的相关指标水平变化。结果：苦参碱可明显延长SCID小鼠生存期，减少外周血白细胞、淋巴细胞、HL-60细胞数量、IL-4、IL-10水平，上调PTEN、IFN-γ、IL-2水平、G0/G1期的细胞含量，与造模组比较差异有统计学意义（P<0.05）。结论：苦参碱对急性髓系白血病HL-60细胞株具有明显的抑制效应，疗效具有一定的剂量依赖性，其作用机制可能与增强PTEN蛋白及调整Th1/Th2细胞平衡有关。

English Summary:

To observe the effects of matrine on the biological characteristics of SCID mice model with acute myeloid leukemia.Methods:A total of 40 mice were randomly divided into a blank group(n=10)and a model group(n=30).The acute myeloid leukemia model was established by intraperitoneal injection of logarithmic HL-60 cells in the model group.4 weeks later，27 mice in the model group were randomly divided into a model group，a matrine A group and a matrine B group，with 9 mice in each group.The rats in group A and B were given 10 mL/kg and 5 mL/kg respectively by gavage，and the rats in the blank group for 4 weeks and model group were given the same volume of normal saline by gavage for 4 weeks.The survival time，body weight，the changes of peripheral blood routine，the level of homologous phosphatase-tensin(PTEN)，the growth of leukemic cell HL-60 and the level of related indexes of Th1/Th2 cells were compared in each group.Results:Matrine could significantly prolong the survival time of SCID mice，decrease the number of leukocytes，lymphocytes，HL-60 cells and the levels of IL-4 and IL-10 in peripheral blood，and increase the levels of PTEN，IFN-γ，IL-2 and G0/G1 cells in the model group.Conclusion:Matrine has obvious inhibitory effects on acute myeloid leukemia HL-60 cell line in a dose-dependent manner，and the curative effects are dependent on dose to some degree.Its mechanism may be related to the enhancement of PTEN protein and regulation of Th1/Th2 cell balance.

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