世界中医药

目的：探索益肾祛浊方对多囊卵巢综合征胰岛素抵抗（PCOS-IR）模型大鼠激素及胰岛素受体表达的干预作用。方法：选取6～8周龄SPF级SD雌性大鼠60只随机分为空白组、模型组、益肾祛浊组、益肾组、西药组（达英-35联合二甲双胍），每组12只。空白组给予普通饲料喂养，其余各组均给予来曲唑灌胃联合高脂饲料喂养至第27天。然后给予不同药物灌胃2周，采用免疫荧光法检测各组大鼠雄激素受体（AR）、促黄体生成素受体（LHR）在卵巢、胃、肠组织的表达；并观察胰岛素受体（INSR）在卵巢的表达。结果：治疗14 d后发现，益肾祛浊方可以显著降低大鼠卵巢局部AR、LHR和INSR的表达，差异有统计学意义（P<0.05）；且明显减少胃中AR及LHR的表达，差异有统计学意义（P<0.05）；同时显著减少结肠组织LHR的表达，差异有统计学意义（P<0.05）。结论：益肾祛浊方可能通过降低AR、LHR表达而调整模型大鼠内分泌紊乱，又可通过降低INSR的表达，对PCOS-IR大鼠的代谢紊异常起到治疗作用。

English Summary:

To explore the intervention effects of Yishen Quzhuo Formula on the expression of hormones and insulin receptors in polycystic ovary syndrome model (PCOS-IR) rats.Methods:A total of 60 SPF level SD female rats aged 6-8 weeks were randomly divided into a blank group, a model group, a Yishen Quzhuo group, a Yishen Quzhuo group, and a Western medicine group (Diane-35 combined with metformin), with 12 rats in each group.The blank group was fed with normal diet, and the other groups were fed with letrozole by gavage combined with high-fat diet until the 27th day.Then, different drugs were fed by gavage for 2 weeks.The expression of androgen receptor (AR), luteinizing hormone receptor (LHR) in ovary, stomach and intestine and insulin receptor (INSR) in ovary were observed by immunofluorescence.Results:After 14 days of treatment, Yishen Quzhuo Formula could significantly reduce the expression of AR, LHR and INSR in part of ovary in rats (P<0.05), the expression of AR and LHR in stomach (P<0.05), and the expression of LHR in colon tissue (P<0.05).Conclusion:Yishen Quzhuo Formula may regulate endocrine disorders in model rats by lowering the expression of AR, LHR and further exert therapeutic effects on metabolic disorder of PCOS-IR rats by reducing the expression level of INSR.

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