| 引用本文:范盎然，于雪，王旭，王淑艳，陈紫微，魏鹏.三七总皂苷对小鼠巨噬细胞炎性反应的影响[J].世界中医药,2020,(08):. |
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| 三七总皂苷对小鼠巨噬细胞炎性反应的影响 |
| Effects of Panax Notoginseng Saponins on Macrophage Inflammation in Mice |
| 投稿时间：2019-06-26 |
| DOI：10.3969/j.issn.1673-7202.2020.08.002 |
| 中文关键词: 巨噬细胞 炎性反应模型 小鼠 三七总皂苷 机制 过氧化物酶体增殖物激活受体-α 炎性调控因子 炎性相关氧化因子 |
| English Keywords:Macrophage Inflammation model Mouse Panax Notoginseng Saponins Mechanism PPAR-α Inflammatory regulatory factor Inflammatory related oxidative factor |
| 基金项目:国家自然科学基金青年基金项目（31800652）;北京中医药大学2018年度基本科研业务费项目（青年教师项目）(2018-JYB-JS004) |
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| 中文摘要: |
| 目的：观察三七总皂苷（PNS）对小鼠巨噬细胞炎性反应的治疗作用，同时探讨其可能机制。方法：通过蛋白质免疫印迹实验、实时PCR法、酶联免疫吸附试验，对照研究PNS对小鼠巨噬细胞系RAW 246.7细胞中炎性反应调控因子过氧化物酶体增殖物激活受体-α(PPAR-α)以及其下游调控的炎性反应递质肿瘤坏死因子-α（TNF-α）、C反应蛋白(CRP)和重组人巨噬细胞集落刺激因子(M-CSF)，白细胞介素6(IL-6)，炎性反应相关氧化因子诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）表达的影响。结果：小鼠巨噬细胞炎性反应模型制备后TNF-α、CRP、M-CSF、IL-6、iNOS及COX-2水平明显上调，三七总皂苷给药后，细胞炎性反应递质与造模组比较有显著降低，差异有统计学意义（P<0.05）。结论：三七总皂苷可明显抑制小鼠巨噬细胞炎性反应效应，其作用机制可能通过激活调控因子PPAR-α实现。 |
| English Summary: |
| To observe the therapeutic effects of Panax notoginseng saponins(PNS)on macrophage inflammation in mice and to explore its possible mechanism.Methods:By Western Blotting test,Real-time PCR method and Elisa test,the effects of PNS on the expression of inflammatory regulatory factors PPAR-α,TNF-α,CRP and M CSF,IL 6,iNOS and COX-2 in mice macrophages were comparatively studied.Results:After the establishment of mouse macrophage inflammation model preparations,the concentrations of TNF-α,CRP,M CSF,IL 6,iNOS and COX-2 were significantly up-regulated.After administration of Panax Notoginseng Saponins,the levels of inflammatory factors were significantly lower than those in the model group,and the differences are statistically significant(P<0.001).Conclusion:Panax notoginseng saponins could significantly inhibit the inflammatory effects of macrophages in mice,and its mechanism may be realized by activating the regulatory factor PPAR-α. |
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