| To observe the effect of Modified Wumei Pill Granule on airway remodeling and TGF-β1/Smad signal pathway in asthma rat model with glucocorticoids intervention.Methods:Totally 30 SD mal rats were randomly divided into the normal group,the asthma group,the dexamethasone group,the budesonide group and the Chinese medicine group.Except for rats in normal group,rat in the other groups were sensitization and stimulation with ovalbumin(OVA)to establish the asthma model.Except for rats in normal and asthma groups,rats in the other groups were treated with dexamethasone and gradually withdrawn.Rats in the budesonide group were inhaled with budesonide.Rats in the Chinese medicine group were poured Modified Wumei Ppill Granule into stomach.Observe the lung histopathological changes under light microscope.Measured the percentage of total wall area(Wat%)and airway smooth muscle area(Wam%).The expression levels of Collagen I and Collagen III in each group were compared by immunohistochemical method.The expression levels of TGF-β1,Smad2,Smad3,Smad7 protein and gene were compared by Western Blotting and RT-PCR.Results:1)Compared with the normal group,the airway wall and smooth muscle layer of asthmatic rats were significantly thicker,the lumen became narrower,Wat% and Wam% increased(P<0.05),and the expression levels of Collagen I,Collagen III,TGF-β1,Smad2,Smad3 protein and gene increased(P<0.05),while the expression level of Smad7 decreased(P<0.05).2)Compared with asthma group,Wat% decreased in dexamethasone group(P<0.05),Collagen I and Collagen III decreased(P<0.05),and TGF-β1,Smad3 protein and gene expression decreased(P<0.05).3)Compared with dexamethasone group,Wat% decreased(P<0.05),Collagen I and Collagen III decreased(P<0.05),and TGF-beta 1,Smad2,Smad3 protein and gene expression decreased(P<0.05).Conclusion:By regulating TGF-β1/Smad signal pathway,Modified Wumei pill granule can reduce the proliferation of airway smooth muscle and the deposition of collagen Ⅰ and collagen Ⅲ protein,inhibit the thickening of airway wall and delay the development of airway remodeling. |
