世界中医药

目的：探究附子理中汤对非酒精性脂肪性肝炎大鼠内毒素及其受体的影响。方法：将75只SD雄性大鼠随机分为3组，分别为正常组，模型组，附子理中汤观察组。采用腹主动脉取血法，收集血液后离心取上清，通过双抗体夹心法检测肿瘤坏死因子-α(TNF-α)和鲎合成基质显色法检测内毒素表达水平；免疫组织化学法检测肝组织内CD14表达水平，并利用逆转录-聚合酶链反应(RT-PCR)法检测Toll样受体-4(TLR-4)与TNF-α mRNA表达水平。结果：与正常组比较，模型组TNF-α水平显著提高，且在第12~24周内持续维持较高水平；附子理中汤观察组TNF-α水平较模型组显著降低，但仍高于正常组；正常组大鼠肝脏TNF-α mRNA未见其表达，模型组TLR4和TNF-α mRNA表达量均显著升高。与模型组比较，附子理中汤观察组TLR4和TNF-α mRNA显著低于模型组，但仍高于正常组TLR4和TNF-α mRNA表达；正常组只有少量细胞能够表达CD14。与正常组比较，模型组大鼠肝组织在第12~24周内，CD14表达的阳性细胞数持续升高，在24周时略有降低，而附子理中汤观察组CD14表达的阳性细胞数与模型组比较显著减少。结论：附子理中汤可降低非酒精性脂肪性肝炎大鼠内毒素及其受体表达。

English Summary:

To explore the effects of Fuzi Lizhong Decoction on endotoxin and its receptor in rats with nonalcoholic steatohepatitis.Methods:A total of 75 SD male rats were randomly divided into 3 groups:a normal group，a model group and a treatment group with Fuzi Lizhong Decoction.Abdominal aortic blood sampling method was used，blood was collected and centrifuged to take supernatant，and the expression level of endotoxin was detected by double antibody filling method to detect tumor necrosis factor alpha(TNF-α)and Limulus synthetic matrix color method was used to detect endotoxin expression; immunohistochemical method was used to detect the expression level of CD14 in liver tissues，and the reverse transcription-polymerase chain reaction(RT-PCR)method was used to detect the expression levels of Toll-like receptor-4(TLR-4)and TNF-α mRNA.Results:Compared with the normal group，the level of TNF-α in the model group increased significantly，and continued to maintain a high level during the 12th to 24th weeks; the level of TNF-α in the Fuzi Lizhong Decoction was significantly lower than that in the model group，but it was still higher than that in the normal group.The expression of TNF-α mRNA in liver of normal group rats was not found，and the expression levels of TLR4 and TNF-α mRNA in model group were significantly increased.Compared with the model group，the TLR4 and TNF-α mRNA of the Fuzi Lizhong Decoction group were significantly lower than the model group，but it was still higher than TLR4 and TNF-α mRNA expression in the normal group; only a small number of cells in the normal group were able to express CD14.Compared with the normal group，the number of CD14-expressing positive cells in the model group's liver tissue continued to increase from 12 to 24 weeks，and slightly decreased at 24 weeks，while the number of CD14-expressing positive cells in the Fuzi Lizhong Decoction group significantly reduced compared with the model group.Conclusion:Fuzi Lizhong Decoction can reduce the expression of endotoxin and its receptor in non-alcoholic steatohepatitis rats.

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