| 引用本文:麦秀云，张静，郭乐琴.黄芪丹参对多囊卵巢综合征大鼠雄激素/胰岛素受体表达影响的研究[J].世界中医药,2020,(20):. |
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| 黄芪丹参对多囊卵巢综合征大鼠雄激素/胰岛素受体表达影响的研究 |
| Effects of Radix Astragali seu Hedysari Combined with Radix Salvia Miltiorrhiza on the Expression of Androgen/Insulin Receptor in Hyperandrogen Anovulatory Rats |
| 投稿时间：2019-05-17 |
| DOI：10.3969/j.issn.1673-7202.2020.20.009 |
| 中文关键词: 多囊卵巢综合征 联合 黄芪 丹参 雄激素受体 胰岛素受体 mRNA 蛋白 |
| English Keywords:Polycystic ovary syndrome Combination Radix Astragali seu Hedysari Radix Salviae Miltiorrhizae Androgen receptor Insulin receptor mRNA Protein |
| 基金项目:2016年广东省中医药局中医药常规科研项目（20161190） |
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| 中文摘要: |
| 目的：研究黄芪联合丹参对多囊卵巢综合征（PCOS）模型大鼠雄激素(AR)及胰岛素受体（INSR）表达的影响。方法：通过对大鼠注射外源性雄激素建立高雄激素无排卵模型，将建模成功的大鼠随机分为黄芪组、丹参组、黄芪＋丹参联合组以及模型组，每组10只，分别以黄芪、丹参、黄芪＋丹参联合用药行不同干预治疗，模型组用生理盐水灌胃。比较各组大鼠血清雄激素、胰岛素水平；卵巢AR、INSR mRNA及蛋白水平。结果：1）与模型组比较，黄芪组血清睾酮水平明显下降，丹参组血清胰岛素水平明显下降；联合组血清睾酮及胰岛素水平均明显下降。2）与模型组比较，黄芪组INSR蛋白水平明显下降，丹参组INSR蛋白水平明显上升；黄芪＋丹参联合组AR蛋白水平明显下降，同时INSR蛋白水平明显升高。3）与模型组比较，黄芪组AR mRNA水平明显下降，丹参组INSR mRNA水平明显上升；黄芪＋丹参联合组AR mRNA水平明显下降，同时卵巢INSR mRNA水平明显升高。结论：黄芪可改善PCOS的高雄激素状态，丹参可改善PCOS的高胰岛素状态，联合用药兼具抗雄激素及降低胰岛素的作用，AR、INSR表达的改变是其可能的作用机制之一。 |
| English Summary: |
| To study the effects of Radix Astragali seu Hedysari and Radix Salviae Miltiorrhizae on the expression of androgen receptor and insulin receptor of rats with polycystic ovary syndrome(PCOS).Methods:The model of hyperandrogen anovulatory was established by injecting exogenous androgen.Rats successfully modeling were randomly divided into an Radix Astragali seu Hedysari group(n＝10),a Radix Salviae Miltiorrhizae group(n＝10),a combined group(n＝10)and a model group(n＝10).The rats were treated with Radix Astragali seu Hedysari,Radix Salviae Miltiorrhizae and Radix Astragali seu Hedysari+Radix Salviae Miltiorrhizae for different interventions.The rats in the model group were given the same dose of saline by gavage,The levels of serum androgen and insulin,ovarian AR,INSR mRNA and protein levels were compared in each group.Results:Compared with the model control group,1)the serum testosterone level was significantly decreased in the Radix Astragali seu Hedysari group,the serum insulin level was significantly decreased in the Radix Salvia Miltiorrhiza group,and the serum testosterone and insulin levels were significantly decreased in the combined group.2)the level of INSR protein in Radix Astragali seu Hedysari group was significantly decreased,the level of INSR protein in Radix Salvia Miltiorrhiza group was significantly increased,the level of AR protein in the combined group was significantly decreased,INSR protein in ovary was significantly increased in combined group.3)the level of ovarian AR mRNA in Radix Astragali seu Hedysari group was significantly decreased,the level of INSR mRNA in Radix Salviae Miltiorrhizae group was significantly increased,and the level of AR mRNA in combined group was significantly decreased while the level of ovarian INSR mRNA was significantly increased.Conclusion:Radix Astragali seu Hedysari can improve the hyperandrogen state of PCOS,Salvia miltiorrhiza can improve the hyperinsulin state of PCOS.Combination medication has anti-androgen and insulin-lowering effects.The change of AR and INSR expression is one of the possible mechanisms. |
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