| 引用本文:麦世瑛1，王怡然1，李永辉1，谭银丰1，张旭光1，张俊清1，李锦云2.胆木化学成分对HUVEC细胞活性氧和通透性的影响[J].世界中医药,2020,(01):. |
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| 胆木化学成分对HUVEC细胞活性氧和通透性的影响 |
| Effects of Chemical Constituents of Nauclea Officinalis on Reactive Oxygen Species and Permeability of HUVEC Cells |
| 投稿时间：2018-12-27 |
| DOI：10.3969/j.issn.1673-7202.2020.01.008 |
| 中文关键词: 胆木 活性氧 通透性 ROS 乌檀 生物碱 酚酸 增殖 |
| English Keywords:Nauclea officinalis Reactive oxygen species Permeability ROS Nauclea Alkaloids Phenolic acid Proliferation |
| 基金项目:海南省自然科学基金项目（20168270）；海南省研究生创新研究项目（Hys2017-182） |
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| 中文摘要: |
| 目的：研究胆木化学成分对HUVEC细胞通透性、ROS生成水平的影响，探讨胆木对HUVEC的保护作用。方法：设有对照组和观察组，通过FITC标记的右旋糖酐（Dextran）试剂盒检测胆木成分对AngⅡ诱导HUVEC单层通透性的影响；以DCFH-DA试剂盒检测胆木化学成分对ROS的抑制作用，采用LPS诱导HUVEC细胞的ROS。结果：胆木生物碱及酚酸类成分对LPS引起的ROS水平增加有抑制趋势，10 μg/mL的喜果苷促进作用有统计学意义（P<0.05）。胆木成分具有促进HUVEC细胞通透性增加的作用，其中异常春花苷内酰胺、喜果苷和原儿茶酸对HUVEC细胞通透性有明显的促进作用。结论：胆木抗炎作用可能与其促进活性氧生成及通透性增加有关。 |
| English Summary: |
| To observe the effects of chemical components from Nauclea officinalis on the HUVEC monolayer permeability and reactive oxygen species(ROS)production induced by pro-inflammatory factors，and to investigate the protective effect of Nauclea officinalis on HUVEC.Methods:A control group and an experimental group were set up.FITC-Dextran kit was used to detect the effects of components from Nauclea officinalis on AngII-induced HUVEC monolayer permeability; DCFH-DA kit was used to detect the inhabitation effects of components from Nauclea officinalis on ROS.The ROS in HUVEC cells were induced by LPS.Results:The results showed that the composition of Nauclea officinalis inhibited the increase of ROS induced by LPS，but the promotion effect of 10 μg/mL of vincosamide was significant.The composition of Nauclea officinalis had the effects of promoting the increase of HUVEC cell permeability.Among them，strictosamid，vincosamiide and protocatechuic acid had significant promoting effects on HUVEC cell permeability.Conclusion:The anti-inflammatory effects of Nauclea officinalis may be related to the promotion of active oxygen production and increased permeability. |
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