| 引用本文:余芝1，庞婷婷1，贾新新2，梁超3，徐斌1.电针大鼠天枢穴抑制西沙必利促进胃运动效应的观察[J].世界中医药,2020,(21):. |
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| 电针大鼠天枢穴抑制西沙必利促进胃运动效应的观察 |
| The Influence of Electroacupuncture at ST25 on Cisapride Induced Gastric Motility with Different Intensity |
| 投稿时间：2020-09-10 |
| DOI：10.3969/j.issn.1673-7202.2020.21.004 |
| 中文关键词: 电针 天枢 针药结合 强度依赖 西沙必利 5-羟色胺 胃运动 胃肠动力障碍 |
| English Keywords:Electroacupuncture ST25 Combination of acupuncture and medicine Intensity-dependence Cisapride Serotonin Gastric motility Gastrointestinal dysmotility |
| 基金项目:国家自然科学基金项目(82074532,81574071,81673883);国家重点基础研究发展计划(973计划)项目(2011CB505206);江苏省第二批中医药领军人才项目(SLJ0225);江苏省中医学优势学科三期项目立项开放课题(ZYX03KF012);河北省自然科学基金项目(H2019402360) |
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| 中文摘要: |
| 目的:采用不同电针强度,观察电针天枢穴对西沙必利诱发的大鼠胃运动的影响,探索电针天枢与西沙必利联合应用的相互作用规律和量效关系。方法:SD大鼠24只,按照随机数字表法,随机分为0.5 mA组,1 mA组和3 mA组,每组8只。在动物胃窦放置球囊,连接压力感受器和多导生理记录系统并记录胃内压,记录基线、干预后(二甲基亚砜溶剂对照、西沙必利和电针)胃内平均压力值和每只大鼠的西沙必利药量。观察电针不同强度天枢结合西沙必利对大鼠胃运动的影响。结果:1)与二甲基亚砜溶剂对照比较,腹腔注射西沙必利后大鼠胃运动明显增强(P<0.01)。2)与注射西沙必利效应比较,采用0.5 mA,1 mA和3 mA的电针强度刺激天枢能明显抑制西沙必利诱发的胃运动增强(P<0.01),并且随着电针强度增加,抑制效应更加明显,0.5 mA和3 mA之间效应差异有统计学意义(P<0.05)。3)计算电针与西沙必利之间的效力关系,在当前药物浓度状态下,0.5 mA天枢产生的效应相当于抑制(1.15±0.24)μg的西沙必利;1 mA相当抑制(3.56±1.31)μg的西沙必利;3 mA相当于抑制(4.03±0.95)μg的西沙必利,0.5 mA和3 mA之间的差异有统计学意义(P<0.05)。结论:不同强度电针大鼠天枢能抑制西沙必利诱发的胃运动增强效应,这一效应呈现强度依赖性。这一结果首次提示,在采用针药结合调节胃运动抑制状态时,选用天枢穴可能会产生拮抗效应,降低药物效应,在选穴时应该慎重考虑。 |
| English Summary: |
| To observe the effect of ST25 combined with cisapride on gastric motility in rats by using different electroacupuncture(EA)intensities,and to explore the law of interaction and efficacy of the combined application of ST25 and cisapride.According to the random number table.Methods:A total of 24 SD rats were randomly divided into 0.5 mA group,1 mA group and 3 mA group,with 8 rats in each group.Placeing a balloon in the gastric antrum of the rats,connecting the baroreceptor and the multi-conduction physiological recording system and record the intragastric pressure,the value of baseline and the average intragastric pressure after intervention(vehicle control,cisapride and EA)was calculated and the amount of cisapride in each rat was recorded to observe the effect of EA at ST25 combined with cisapride on gastric motility in rats.Results:1)Compared with the vehicle control,the gastric movement of rats was significantly enhanced after intraperitoneal injection of cisapride(P<0.01).2)Compared with the cisapride,the stimulation of EA at ST25 with 0.5 mA,1 mA and 3 mA could significantly inhibit the gastric movement induced by cisapride(P<0.01),and as the intensity of the EA increases,the suppression effect was more obvious.The difference in effect between 0.5 mA and 3 mA was statistically significant(P<0.05).3)The efficacy relationship between EA and cisapride was calculated.At the current concentration,the effect of 0.5 mA ST25 is equivalent to(1.15±0.24)μg of cisapride; 1 mA is equivalent to(3.56±1.31)μg of cisapride; 3 mA is equivalent to(4.03±0.95)μg of cisapride.There was a statistically significant difference in the effect between 0.5 mA and 3 mA(P<0.05).Conclusion:EA at ST25 with different intensities can inhibit the enhanced gastric motility induced by cisapride in rats,which is intensity-dependent. |
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