| 引用本文:王浩，邹茜.人参皂苷Rg1调节沉默信息调节因子1对抗晶状体上皮细胞衰老的影响[J].世界中医药,2021,(07):. |
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| 人参皂苷Rg1调节沉默信息调节因子1对抗晶状体上皮细胞衰老的影响 |
| Ginsenoside Rg1 Prevent Senescence of Lens Epithelial Cells By Regulates Sirt1 |
| 投稿时间：2019-05-19 |
| DOI：10.3969/j.issn.1673-7202.2021.07.013 |
| 中文关键词: 年龄相关性白内障 晶状体上皮细胞 人参皂苷Rb1 沉默信息调节因子1 细胞衰老 凋亡 炎证 氧化损伤 |
| English Keywords:Age-related Cataract Lens Epithelial Cells Ginsenoside Rb1 SIRT1 Cell senescence Apoptosis Inflammation Oxidative damage |
| 基金项目:国家自然科学基金项目（8187040994）；常州市科技计划项目（CJ20190062） |
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| 中文摘要: |
| 目的：观察人参皂苷Rb1是否通过调节沉默信息调节因子1（SIRT1）表达抗晶状体上皮细胞衰老。方法：构建晶状体上皮细胞株SRA01/04衰老模型，加入人参皂苷Rb1培养后通过PCR检测SIRT1的表达改变，通过β-半乳糖苷酶染色检测衰老情况。敲降正常晶状体上皮细胞株SRA01/04中SIRT1的表达后检测衰老情况，再次加入人参皂苷Rb1后通过PCR和免疫荧光观察上述指标有无改变。结果：60 μmol/L人参皂苷Rb1处理衰老SRA01/04细胞内SIRT1表达增加，差异有统计学意义(P<0.05)；SIRT1沉默后，SRA01/04细胞衰老程度明显增加，差异有统计学意义(P<0.05)；与SIRT1沉默组比较，SIRT1沉默同时加入人参皂苷Rb1后，SIRT1有所上升。结论：人参皂苷Rb1可以通过调节SIRT1表达抗晶状体上皮细胞衰老。 |
| English Summary: |
| To observe whether ginsenoside Rb1 can inhibit senescence of lens epithelial cells by regulating expression of SIRT1.Methods:Senescence model of lens epithelial cell line SRA01/04 was established,and cultured with adding ginsenoside Rb1.The mRNA expression of SIRT1 was detected by PCR and the degree of senescence were detected by β-galactosidase staining positive rate.The expression of SIRT1 in the normal lens epithelial cell line SRA01/04 was knocked down to detect aging,and then ginsenoside Rb1 was added again to observe whether the above indicators have changed by PCR and immunofluorescence.Results:60 μmol/L ginsenoside Rb1 treatment of senescent SRA01/04 cells increased the expression of SIRT1 (P<0.05); after silencing SIRT1,SRA01/04 cells senescence significantly increased (P<0.05); compared with the silence SIRT1 group,after silencing SIRT1 simultaneously adding ginsenoside Rb1,SIRT1 increased.Conclusion:Ginsenoside Rb1 can resist the senescence of lens epithelial cells by regulating the expression of SIRT1. |
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