| 引用本文:蒋涛1,2，孔博1,2，颜威1,2，吴昌桂1,2，徐醒2，江敏2，奚小冰1,2.基于网络药理学研究络石藤-伸筋草药对治疗骨关节炎的作用机制[J].世界中医药,2020,(24):. |
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| 基于网络药理学研究络石藤-伸筋草药对治疗骨关节炎的作用机制 |
| Potential Mechanism of Herb Pair Caulis Trachelospermi and Herba Lycopodii on Osteoarthritis Based on the Network Pharmacology Study |
| 投稿时间：2020-02-25 |
| DOI：10.3969/j.issn.1673-7202.2020.24.003 |
| 中文关键词: 网络药理学 络石藤 伸筋草 药对 骨关节炎 |
| English Keywords:Network pharmacology Caulis Trachelospermi Herba Lycopodii Herb pair Osteoarthritis |
| 基金项目:国家自然科学基金资助项目（81703576）；上海市中医药三年行动计划项目［ZY-（2018-2020）-CCCX-1011］ |
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| 中文摘要: |
| 目的：运用网络药理学方法研究络石藤-伸筋草药对治疗骨关节炎的生物活性成分和潜在的作用机制。方法：从中药系统药理学数据库和分析平台（TCMSP）和中国科学院化学专业数据库（CASC）中收集已知的络石藤-伸筋草药对中的生物活性化学成分；使用Swiss Target Prediction网络服务器预测化合物的蛋白靶点；从疾病数据库中挖掘已知的骨关节炎相关靶点数据；利用收集到的数据分别构建蛋白质-蛋白质相互作用（PPI）网络并提取其交集作为药物-疾病相互作用网络，通过网络拓扑学分析筛选出核心靶点；使用DAVID数据库对核心靶点进行GO注释分析和KEGG通路分析。结果：从TCMSP和CASC数据库中共收集到108个已知的络石藤-伸筋草药对中的化学成分，虚拟筛选后得到17个生物活性化合物，并预测出117个潜在的蛋白靶点；从疾病数据库中一共检索到191个骨关节炎相关靶点；对化合物和疾病的交集PPI网络进行网络拓扑学分析得到208个核心靶点，这些靶点主要富集到自噬、凋亡、炎性反应、雌激素相关的生物过程和信号通路。结论：络石藤-伸筋草药对可能通过多条生物途径发挥调节炎性反应、自噬、凋亡和类雌激素样作用发挥抗骨关节炎作用。 |
| English Summary: |
| To excavate the bio-active compounds and potential molecular mechanism for the herb pair Caulis Trachelospermi and Herba Lycopodii on osteoarthritis (OA) based on the network pharmacology method.Methods:Known bioactive-ingredients of the of herb pair were obtained from the Chinese Medicine System Pharmacology Database and Analysis Platform (TCMSP) and the Shanghai Institute of Organic Chemistry of CAS，Chemistry Database (CASC) with their potential targets predicted by the Swiss Target Prediction server.Known OA-related genes were mined in disease databases; protein-protein interaction (PPI) network was constructed with the collected data，and their intersection was extracted as drug-disease interaction network; core gene targets were screened through network topology analysis.Kyoto Encyclopedia of Genes and Genomes signaling pathway analysis and Gene ontology analysis were performed to reveal gene function by DAVID database.Results:A total of 108 ingredients of two herbs were retrieved from the TCMSP and CASC databases and 17 of them are considered to be bio-active which yield 117 possible targets.191 OA-related targets were retrieved from the disease database.Network topology analysis of the Drug-disease interaction network yielded 208 core targets，which mainly enriched into inflammation，autophagy，and estrogen-related biological processes and pathways.Conclusion:The Caulis Trachelospermi-Herba Lycopodii herb pair may treat OA through adjusting inflammatory，autophagy and exerting estrogen-like effect，which embodied the multi-component，multi-target and multi-pathway synergy characteristics of traditional Chinese medicine，and provided ideas for further research. |
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