| 引用本文:李朋1，李钊1，罗天1，胡仕畅2，霍少川3.绝经后妇女不同中医证型膝骨关节炎关节软骨ERRα/SOX9信号通路表达分析[J].世界中医药,2021,(02):. |
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| 绝经后妇女不同中医证型膝骨关节炎关节软骨ERRα/SOX9信号通路表达分析 |
| Analysis of ERR a /SOX9 Signaling Pathway in Articular Cartilage of Postmenopausal with Different Traditional Chinese Medicine Syndromes of Knee Osteoarthritis |
| 投稿时间：2019-12-10 |
| DOI：10.3969/j.issn.1673-7202.2021.02.027 |
| 中文关键词: 膝骨关节炎 ERRα/SOX9信号通路 绝经后妇女 关节软骨 中医辨证分型 |
| English Keywords:Knee Osteoarthritis ERR a /SOX9 signaling pathway Postmenopausal Articular cartilage TCM syndrome differentiation |
| 基金项目:广东省中医药局科研项目(20181140) |
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| 中文摘要: |
| 目的:探讨ERRα/SOX9信号通路在绝经后妇女不同证型膝骨关节炎(KOA)关节软骨中的作用。方法:选取2018年6月至2019年4月广州中医药大学第三附属医院收治的绝经后KOA患者,根据中医证型标准分为风寒湿痹证、风湿热痹证、瘀血闭阻证和肝肾亏虚证4组,采用RT-PCR和Western Blot检测ERRα/SOX9信号通路表达情况。结果:各组间膝关节软骨组织ERRα、SOX9和Col2α1表达量比较差异有统计学意义(P<0.05)。与风寒湿痹组比较,风湿热痹组ERRα、SOX9和Col2α1表达量之间差异无统计学意义(P>0.05);瘀血痹阻组ERRα表达量升高,SOX9、Col2α1表达量降低(P<0.05);肝肾亏虚组ERRα、Col2α1表达量降低(P<0.05),SOX9表达量升高(P<0.05)。与风湿热痹组比较,瘀血痹阻组ERRα表达量升高,SOX9、Col2α1表达量降低(P<0.05);肝肾亏虚组ERRα、Col2α1表达量降低(P<0.05),SOX9表达量升高(P<0.05)。与瘀血痹阻组比较,肝肾亏虚组ERRα、Col2α1表达量降低(P<0.05);SOX9表达量升高(P<0.05)。结论:绝经后妇女不同中医证型KOA患者关节软骨组织ERRα/SOX9信号通路存在差异,揭示不同证型ERRα/SOX9信号通路的差异将为中医药辨证施治绝经后KOA提供理论基础。 |
| English Summary: |
| To investigate the role of ERRa /SOX9 signaling pathway in postmenopausal women with different types of knee osteoarthritis articular cartilage.Methods:Postmenopausal KOA patients admitted to Third Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine were selected as the research objects and were divided into 4 groups according to TCM syndrome type criteria:wind-cold-dampness arthralgia syndrome，wind-dampness-heat arthralgia，blood stasis arthralgia syndrome，and liver-kidney deficiency syndrome.Rt-pcr and Western Blot were used to detect the ERR alpha /SOX9 signal pathway expression.Results:The difference of ERR-alpha，SOX9 and Col2-alpha-1 expression was statistically significant(P<0.05).Compared with wind-cold-dampness arthralgia group，there was no statistically significant difference in the expression of ERRα，SOX9 and Col2α1 in wind-dampness-heat arthralgia groups(P<0.05).Compared with the blood stasis arthralgia瘀血痹阻 group，there was no significant difference in the expression of ERRα，SOX9 and Col2α1 in the wind-dampness-heat arthralgia group(P>0.05); the expression of ERRα increased in the blood stasis arthralgia group，and the expression of SOX9 and Col2α1 decreased(P<0.05); the expression of ERRα and Col2α1 in the liver and kidney deficiency group decreased(P<0.05)，and the expression of SOX9 increased(P<0.05).Compared with the wind-dampness-heat arthralgia，the expression of ERRα in the blood stasis arthralgia increased，and the expression of SOX9 and Col2α1 decreased(P<0.05); the expression of ERRα and Col2α1 in the liver and kidney deficiency group decreased(P<0.05)，and the expression of SOX9 increased(P<0.05).Compared with blood stasis arthralgia group，the expression of ERRα and Col2α1 in the liver and kidney deficiency group decreased(P<0.05); the expression of SOX9 increased(P<0.05).Conclusion:There are differences in the ERR alpha /SOX9 signaling pathways in the articular cartilage of postmenopausal women with different TCM syndromes of KOA.The discovery of the differences in the ERR alpha /SOX9 signaling pathways between different syndroms of KOA provides a theoretical basis for TCM syndrome differentiation in the treatment of post-menopausal KOA. |
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