| 引用本文:祝雪丽1，熊尚全3，陈怀宇3，褚剑锋2，吴翔1，何绍吾1，李玮1.丹参饮介导过氧化物酶体增殖物激活型受体γ减轻大鼠冠脉粥样硬化[J].世界中医药,2021,(11):. |
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| 丹参饮介导过氧化物酶体增殖物激活型受体γ减轻大鼠冠脉粥样硬化 |
| The Mechanism Study of Danshen Decoction Mediated PPAR-γ to Reduce Coronary Atherosclerosis in Rats |
| 投稿时间：2021-04-28 |
| DOI：10.3969/j.issn.1673-7202.2021.11.010 |
| 中文关键词: 丹参饮 冠状动脉粥样硬化 过氧化物酶体增殖物激活型受体γ 肝X受体α 三磷酸腺苷结合盒转运体A1 丹参 檀香 砂仁 冠心病预防 |
| English Keywords:Danshen Drink Coronary atherosclerosis PPAR-γ ABCA1 LXR-α Radix Salviae Miltiorrhizae Lignum Santali Albi Fructus Amomi Villosi Prevention of coronary heart disease |
| 基金项目:福建省教育厅中青年教师教育科研项目（JAT170287）；福建省创双高重点专科项目（闽卫中医函2017-881） |
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| 中文摘要: |
| 目的：研究丹参饮通过介导过氧化物酶体增殖物激活型受体γ（PPAR-γ）/肝X受体α（LXR-α）/三磷酸腺苷结合盒转运体A1（ABCA1）信号通路减轻大鼠冠状动脉粥样硬化的作用机制。方法：选取无特定病原体(SPF)级40只健康Wistar大鼠作为实验动物，随机分为空白组、冠状动脉粥样硬化组（对照组）、丹参饮低剂量观察组（低剂量组）和丹参饮高剂量观察组（高剂量组），比较各组大鼠的炎症介质水平、ICAM-1水平、脂代谢水平、PPAR-γ、LXR-α和ABCA1 mRNA表达和蛋白表达。结果：对照组、低剂量组和高剂量组的三酰甘油（TG）、胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）和低密度脂蛋白胆固醇（LDL-C）均高于空白组（P<0.05），低剂量组和高剂量组的TG、TC和LDL-C均低于对照组（P<0.05），且高剂量组低于低剂量组（P<0.05），但4组的HDL-C表达差异无统计学意义（P>0.05）；对照组、低剂量组和高剂量组的炎症介质和ICAM-1表达均高于空白组（P<0.05），低剂量组和高剂量组的炎症介质和ICAM-1表达均低于对照组（P<0.05），且高剂量组低于低剂量组（P<0.05）；对照组、低剂量组和高剂量组的PPAR-γ、LXR-α和ABCA1 mRNA和蛋白表达均高于空白组（P<0.05），且低剂量组和高剂量组明显高于对照组（P<0.05），高剂量组明显高于低剂量组（P<0.05）。结论：丹参饮可以通过对PPAR-γ-LXR-α-ABCA1信号通路的介导，发挥出抗冠状动脉粥样硬化的作用。 |
| English Summary: |
| To study on Danshen Drink by mediating peroxisome proliferator-activated receptor γ(PPAR-γ)/liver X receptor α(LXR-α)/adenosine triphosphate binding cassette transporter A1(ABCA1) signaling pathway to alleviate the mechanism of coronary atherosclerosis of rats.Methods:A total of 40 healthy Wistar rats of SPF-free grade were selected as the experimental animals，and were randomly divided into a blank group，a coronary atherosclerosis group(control group)，Danshen Drink low-dose treatment group(low-dose group) and Danshen Drink high Dose treatment group(high-dose group).The levels of inflammatory mediators，ICAM-1，lipid metabolism，PPAR-γ，LXR-α and ABCA1 mRNA expression and protein expression in each group of rats were compared.Results:The triacylglycerol(TG)，cholesterol(TC)，high-density lipoprotein cholesterol(HDL-C) and low-density lipoprotein cholesterol(LDL-C) of the control group，low-dose group and high-dose group were all higher than those of the blank group(P<0.05)，the TG，TC and LDL-C of the low-dose group and the high-dose group were lower than those of the control group(P<0.05)，and the high-dose group was lower than the low-dose group(P<0.05)，but there was no statistically significant difference in the HDL-C expression of 4 groups(P>0.05); the expressions of inflammatory mediators and ICAM-1 in the control group，low-dose group and high-dose group were higher than those in the blank group(P<0.05)，low-dose group and high-dose group The expressions of inflammatory mediators and ICAM-1 in the control group were lower than those in the control group(P<0.05).The protein expressions of PPAR-γ、LXR-α and ABCA1 mRNA were higher than those of the blank group(P<0.05).And the low-dose and high-dose groups were significantly higher than those of the control group(P<0.05)，and the high-dose group was significantly higher than the low-dose group(P<0.05).Conclusion:Danshen Drink can play an anti-coronary atherosclerosis effect by mediating the PPAR-γ-LXR-α-ABCA1 signaling pathway. |
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