| 引用本文:邓樱，唐润伟，卫菊，乐枫，刘云龙，钱耀明.“黄芪-莪术”药对通过PTEN与p-AKT对人乳腺癌细胞增殖的影响[J].世界中医药,2021,(11):. |
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| “黄芪-莪术”药对通过PTEN与p-AKT对人乳腺癌细胞增殖的影响 |
| Effects of “Radix Astragali seu Hedysari-Rhizoma Curcumae” on Proliferation of MDA-MB-231 Cells through PTEN and p-AKT |
| 投稿时间：2020-08-27 |
| DOI：10.3969/j.issn.1673-7202.2021.11.013 |
| 中文关键词: 黄芪 莪术 药对 MDA-MB-231 增殖 PTEN p-AKT 乳腺癌 |
| English Keywords:Radix Astragali seu Hedysari Rhizoma Curcumae,Herb-partners MDA-MB-231 Proliferation PTEN p-AKT Breast cancer |
| 基金项目:上海交通大学医学院医工交叉项目（0507N17055） |
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| 中文摘要: |
| 目的：探讨“黄芪-莪术”药对通过PTEN与p-AKT对人乳腺癌细胞（MDA-MB-231）增殖的作用机制。方法：将MDA-MB-231细胞进行“黄芪-莪术”药对（药对组）、顺铂（顺铂组）、“黄芪-莪术”药对联合顺铂（药对+顺铂组）治疗，并设置空白对照组。MTT法检测细胞增殖，RT-qPCR检测PTEN与p-AKT的mRNA表达水平，Western Blotting检测p-AKT蛋白表达水平。结果：与空白对照组比较，药对组PTEN mRNA表达水平上升，OD值与p-AKT蛋白表达水平下降（P<0.05），p-AKT mRNA蛋白表达水平变化差异无统计学意义（P>0.05）；顺铂组OD值与PTEN mRNA表达水平下降（P<0.05），p-AKT mRNA与蛋白表达水平变化差异无统计学意义（P>0.05）；而药对+顺铂组在药对治疗的基础上能进一步抑制MDA-MB-231细胞增殖，促进PTEN mRNA的表达，抑制p-AKT mRNA与蛋白表达水平。结论：“黄芪-莪术”药对抑制MDA-MB-231细胞增殖的机制可能通过上调PTEN基因而直接抑制AKT蛋白水平。 |
| English Summary: |
| To explore the effects mechanism of “Radix Astragali seu Hedysari-Rhizoma Curcumae” on the proliferation of MDA-MB-231 cells through PTEN and p-AKT.Methods:MDA-MB-231 cells were treated with “Radix Astragali seu Hedysari-Rhizoma Curcumae” drug pair(drug pair group),cisplatin(cisplatin group),“Radix Astragali seu Hedysari-Rhizoma Curcumae” drug pair combined with cisplatin(drug pair+cisplatin group),and blank control group was set.Then,MTT method was used to detect cell proliferation,RT-qPCR was used to detect PTEN and p-AKT mRNA expression levels,and Western blot was used to detect p-AKT protein expression levels.Results:Compared with the blank control group,the PTEN mRNA expression level of the drug-pair group increased,the OD value and p-AKT protein expression level decreased(P<0.05),the p-AKT mRNA protein expression level did not change significantly(P>0.05); the OD value and PTEN mRNA expression level of the cisplatin group decreased(P<0.05),and the expression levels of p-AKT mRNA and protein did not change significantly(P>0.05); while the drug pair+cisplatin group could further improve on the basis of drug pair treatment Inhibit MDA-MB-231 cell proliferation,promote PTEN mRNA expression,and inhibit p-AKT mRNA and protein expression levels.Conclusion:The mechanism of “Radix Astragali seu Hedysari-Rhizoma Curcumae” drug on inhibiting MDA-MB-231 cell proliferation may directly inhibit AKT protein level by up-regulating PTEN gene. |
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