| 引用本文:王振涛1，刘金钊2.17β-雌二醇及补骨脂素对骨髓间充质细胞向成骨细胞分化的诱导作用[J].世界中医药,2021,(13):. |
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| 17β-雌二醇及补骨脂素对骨髓间充质细胞向成骨细胞分化的诱导作用 |
| Induction of Differentiation of Bone Marrow Mesenchymal Cells into Osteoblasts by 17 Beta Estradiol Combined with Psoralen |
| 投稿时间：2019-02-14 |
| DOI：10.3969/j.issn.1673-7202.2021.13.010 |
| 中文关键词: 骨髓间充质干细胞 17β-雌二醇 补骨脂素 成骨细胞 成骨相关基因RUNX2 细胞分化 诱导作用 Ⅰ型胶原 |
| English Keywords:Bone marrow mesenchymal stem cells 17 beta estradiol Psoralen Osteoblast Osteogenic related gene RUNX2 Cell differentiation Induction Type I Collagen |
| 基金项目:山东省医药卫生科技发展计划(保健项目)项目(2013BJYB05) |
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| 中文摘要: |
| 目的:观察17β-雌二醇对骨髓间充质干细胞向成骨细胞分化的诱导作用。方法:将大鼠的骨髓间充质干细胞(BMSCs)暴露于不同浓度的17β-雌二醇、补骨脂素增殖和成骨分化培养基。通过茜素红(Alizarin Red)染色法观察细胞增殖;通过酶联免疫吸附试验法(ELISA)和实时聚合酶链反应(PCR)评估成骨标志物Ⅰ型胶原的分泌水平以及关键因子RUNX2的表达情况。结果:与对照组比较,17β-雌二醇补充剂促进BMSCs增殖。BMSCs增殖添加的17β-雌二醇的不同剂量(0、0.001、0.01、0.1 nmol/L),有效地改善了骨髓间充质干细胞分泌Ⅰ型胶原的水平,RUNX2的mRNA也被上调。17β-雌二醇干预后第5天，Ⅰ型胶原表达显著升高(P<0.05),在17β-雌二醇干预的第7天,RUNX2 mRNA及蛋白表达随17β-雌二醇水平的增加而升高(P<0.05)。结论:17β-雌二醇联合补骨脂素可以作为一种有效的调节剂，以提高骨髓再生中MSC的能力。 |
| English Summary: |
| To observe the inducing effects of 17β-estradiol on the differentiation of bone marrow mesenchymal stem cells into osteoblasts.Methods:BMSCs from rat bone marrow were exposed to different concentrations of 17 beta-estradiol(E2) and psoralen proliferation and osteogenic differentiation media.Cell proliferation was observed by Alizarin Red staining,and the secretion of collagen type I and the expression of key factor RUNX2 were evaluated by ELISA and real-time PCR.Results:17 β-estradiol supplements promoted the proliferation of BMSCs compared with the control group.The different doses of 17 β-estradiol(0,0.001,0.01,0.1 nmol/L) added to the proliferation of BMSCs effectively improved the level of type I collagen secreted by bone marrow mesenchymal stem cells,and the mRNA of RUNX2 was also up-regulated.On the 5th after β-estradiol intervention,the expression of type I collagen was significantly increased(P<0.05).On the 7th day of 17β-estradiol intervention,RUNX2 factor mRNA and protein expression increased with the increase of 17 β-estradiol concentration(P<0.05).Conclusion:17 beta-estradiol combined with psoralen can be used as an effective regulator to improve the ability of MSCs in bone marrow regeneration. |
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