| 引用本文:宁娜1,刘珊珊2,黄秋凌2,李燕舞3,彭丹婷1.虚汗停颗粒对环磷酰胺模型大鼠免疫功能及肠道菌群的影响[J].世界中医药,2021,(16):. |
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| 虚汗停颗粒对环磷酰胺模型大鼠免疫功能及肠道菌群的影响 |
| Effects of Xuhanting Granule on Immune Function and Intestinal Microflora of Cyclophosphamide Model Rats |
| 投稿时间：2021-07-07 |
| DOI：10.3969/j.issn.1673-7202.2021.16.009 |
| 中文关键词: 虚汗停颗粒 免疫调控 环磷酰胺 匹多莫德颗粒 免疫细胞因子 16S rRNA基因测序 脾脏病理形态 肠道菌群 |
| English Keywords:Xuhanting Granules Immunoregulation Cyclophosphamide Pidotimod granule Immune cell molecule 16S rRNA gene sequencing Pathological form of spleen Intestinal flora |
| 基金项目:广东省教育厅重点领域项目（2020ZDZX3040）——基于智能可穿戴设备数据探讨潮热特征人群的运动-代谢-菌群网络研究 |
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| 中文摘要: |
| 目的：探讨虚汗停颗粒对环磷酰胺模型大鼠免疫功能及肠道菌群的干预作用。方法：将SPF级SD大鼠随机分为5组，分别为正常对照组、环磷酰胺模型组、虚汗停低剂量组（1.7 g/kg）、高剂量组（3.4 g/kg）、匹多莫德组（0.23 g/kg）。除正常对照组外，其他各组大鼠采用环磷酰胺腹腔注射制备免疫功能抑制模型。用流式细胞计数法检测各组大鼠外周血淋巴细胞分类，ELISA法检测血清免疫球蛋白IgG、IgM及补体C3、C4含量，16S rDNA测序检测大鼠肠道菌群变化。常规固定大鼠脾脏，HE染色后光镜下进行形态学观察。结果：环磷酰胺模型大鼠外周血淋巴细胞中CD3、CD4、CD8细胞比例明显降低，虚汗停高剂量组及匹多莫德组对环磷酰胺模型大鼠外周血淋巴细胞中CD3细胞比例有明显提高，其他各组均无显著变化。模型大鼠血清中IgG、IgM、C4含量明显降低，虚汗停低剂量组及匹多莫德组模型大鼠血清IgG、IgM含量均显著升高。肠道菌群检测结果显示，模型大鼠菌群结构与正常大鼠菌群明显不同；药物干预各组与模型组微生物结构的差异均有统计学意义（均P<0.01）。结论：虚汗停颗粒可以调节环磷酰胺模型大鼠肠道菌群结构，改善部分免疫功能及脾脏病理形态，体现了中药复方多靶点效应，为临床应用提供了实验依据。 |
| English Summary: |
| To explore the effect of Xuhanting Granules on immune function and intestinal microflora of cyclophosphamide rats. Methods:A total of 50 rats were randomly divided into 5 groups:a normal control group, a cyclophosphamide model group, Xuhanting low, high dose groups (1.7 g/kg, 3.4 g/kg), and a pidotimod group (0.23g/kg). Except for the normal control group, the immunosuppressive model was established by intraperitoneal injection of cyclophosphamide. Flow cytometry was used to detect the classification of peripheral blood lymphocytes of rats, and ELISA method was used to detect the contents of serum immunoglobulin IgG, IgM and complement C3 and C4. 16S rDNA sequencing was used to detect the changes of intestinal flora in rats. The spleen of rats was fixed routinely, and morphological observation was carried out under light microscope after HE staining. Results:The proportion of CD3, CD4 and CD8 cells in peripheral blood lymphocytes of cyclophosphamide model rats was significantly decreased, and the proportion of CD3 cells in peripheral blood lymphocytes of rats with cyclophosphamide was significantly increased by xuhanding 3.4g/kg and pidotimod group. The contents of IgG, IgM and C4 in the serum of model rats were significantly decreased. The contents of IgG, IgM and C4 in serum of model rats were significantly increased by Xuhanting 1.7 g/kg and pidotimod. The results of intestinal microflora detection showed that the microflora structure of model rats was significantly different from that of normal rats, and there were significant differences in the microflora structure between the drug intervention groups and the model group (P<0.01).Conclusion:Xuhanting Granules can regulate the structure of intestinal microflora and improve partial immune function and pathological morphology of spleen in cyclophosphamide model rats, reflecting the multi-target effect of traditional Chinese medicine compound, and provide experimental basis for clinical application. |
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